Articles | Volume 16, issue 22
https://doi.org/10.5194/acp-16-14079-2016
© Author(s) 2016. This work is distributed under
the Creative Commons Attribution 3.0 License.
the Creative Commons Attribution 3.0 License.
https://doi.org/10.5194/acp-16-14079-2016
© Author(s) 2016. This work is distributed under
the Creative Commons Attribution 3.0 License.
the Creative Commons Attribution 3.0 License.
Research article
| 
15 Nov 2016
Research article |
| 15 Nov 2016
In vitro exposure to isoprene-derived secondary organic aerosol by direct deposition and its effects on COX-2 and IL-8 gene expression
Maiko Arashiro
Department of Environmental Sciences and Engineering, Gillings School
of Global Public Health, University of North Carolina at Chapel Hill, Chapel
Hill, NC 27599, USA
Ying-Hsuan Lin
Department of Environmental Sciences and Engineering, Gillings School
of Global Public Health, University of North Carolina at Chapel Hill, Chapel
Hill, NC 27599, USA
Michigan Society of Fellows, Department of Chemistry, University of
Michigan, Ann Arbor, MI 48109, USA
Kenneth G. Sexton
Department of Environmental Sciences and Engineering, Gillings School
of Global Public Health, University of North Carolina at Chapel Hill, Chapel
Hill, NC 27599, USA
Zhenfa Zhang
Department of Environmental Sciences and Engineering, Gillings School
of Global Public Health, University of North Carolina at Chapel Hill, Chapel
Hill, NC 27599, USA
Ilona Jaspers
Department of Environmental Sciences and Engineering, Gillings School
of Global Public Health, University of North Carolina at Chapel Hill, Chapel
Hill, NC 27599, USA
Center for Environmental Medicine, Asthma, and Lung Biology, School
of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC
27599, USA
Curriculum in Toxicology, University of North Carolina at Chapel
Hill, Chapel Hill, NC 27599, USA
Department of Pediatrics, School of Medicine, University of North
Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
Department of Microbiology and Immunology, School of Medicine,
University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
Rebecca C. Fry
Department of Environmental Sciences and Engineering, Gillings School
of Global Public Health, University of North Carolina at Chapel Hill, Chapel
Hill, NC 27599, USA
Curriculum in Toxicology, University of North Carolina at Chapel
Hill, Chapel Hill, NC 27599, USA
William G. Vizuete
Department of Environmental Sciences and Engineering, Gillings School
of Global Public Health, University of North Carolina at Chapel Hill, Chapel
Hill, NC 27599, USA
Avram Gold
Department of Environmental Sciences and Engineering, Gillings School
of Global Public Health, University of North Carolina at Chapel Hill, Chapel
Hill, NC 27599, USA
Department of Environmental Sciences and Engineering, Gillings School
of Global Public Health, University of North Carolina at Chapel Hill, Chapel
Hill, NC 27599, USA
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Fine particulate matter (PM2.5) has become increasingly important to regulate and model. In this study, we parameterize non-ideal aerosol mixing and phase state into the Community Multiscale Air Quality (CMAQ) model and analyze its impact on the formation of a globally important source of PM2.5, isoprene epoxydiol (IEPOX)-derived PM2.5. Incorporating these features furthers model bias in IEPOX-derived PM2.5, however, this work provides potential phase state bounds for future PM2.5 modeling work.
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Nahin Ferdousi-Rokib, Stephanie Jacoby, N. Cazimir Armstrong, Alana J. Dodero, Martin Changman Ahn, Ergine Zephira Remy, Zhenfa Zhang, Avram Gold, Joseph L. Woo, Yue Zhang, Jason D. Surratt, and Akua A. Asa-Awuku
Atmos. Chem. Phys., 25, 15613–15630, https://doi.org/10.5194/acp-25-15613-2025, https://doi.org/10.5194/acp-25-15613-2025, 2025
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This study looks at how natural compounds when mixed with salts in the air affect how clouds form. These compounds come from plants and are found all over the world. They are sticky, and this changes how water droplets and clouds form. Sometimes the compound spreads more easily when mixed with salt, while the other compound does not change. Depending on the condition, these mixtures can behave differently, which affects how we predict cloud formation.
Christopher N. Rapp, Sining Niu, N. Cazimir Armstrong, Xiaoli Shen, Thomas Berkemeier, Jason D. Surratt, Yue Zhang, and Daniel J. Cziczo
Atmos. Chem. Phys., 25, 5519–5536, https://doi.org/10.5194/acp-25-5519-2025, https://doi.org/10.5194/acp-25-5519-2025, 2025
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Atmospheric ice formation is initiated by particulate matter suspended in air and has profound impacts on Earth's climate. This study focuses on examining the effectiveness of ice formation by a subset of particles composed of organic matter and sulfate. We used experiments and computer modeling to obtain the result that these particles are not effective ice-nucleating particles, suggesting that molecular structure is important for ice formation on these types of particles.
Timur Cinay, Dickon Young, Nazaret Narváez Jimenez, Cristina Vintimilla-Palacios, Ariel Pila Alonso, Paul B. Krummel, William Vizuete, and Andrew R. Babbin
Atmos. Chem. Phys., 25, 4703–4718, https://doi.org/10.5194/acp-25-4703-2025, https://doi.org/10.5194/acp-25-4703-2025, 2025
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Sara L. Farrell, Havala O. T. Pye, Robert Gilliam, George Pouliot, Deanna Huff, Golam Sarwar, William Vizuete, Nicole Briggs, Fengkui Duan, Tao Ma, Shuping Zhang, and Kathleen Fahey
Atmos. Chem. Phys., 25, 3287–3312, https://doi.org/10.5194/acp-25-3287-2025, https://doi.org/10.5194/acp-25-3287-2025, 2025
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In this work we implement heterogeneous sulfur chemistry into the Community Multiscale Air Quality (CMAQ) model. This new chemistry accounts for the formation of sulfate via aqueous oxidation of SO2 in aerosol liquid water and the formation of hydroxymethanesulfonate (HMS) – often confused by measurement techniques as sulfate. Model performance in predicting sulfur PM2.5 in Fairbanks, Alaska, and other places that experience dark and cold winters is improved.
Ningjin Xu, Chen Le, David R. Cocker, Kunpeng Chen, Ying-Hsuan Lin, and Don R. Collins
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Hazardous air pollutant (HAP) human exposure studies usually rely on local measurements or dispersion model methods, but those methods are limited under spatial and temporal conditions. We processed the US EPA emission data to simulate the hourly HAP emission patterns and applied the chemical transport model to simulate the HAP concentrations. The modeled HAP results exhibit good agreement (R is 0.75 and NMB is −5.6 %) with observational data.
Molly Frauenheim, Jason D. Surratt, Zhenfa Zhang, and Avram Gold
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We report synthesis of the isoprene-derived photochemical oxidation products trans- and cis-β-epoxydiols in high overall yields from inexpensive, readily available starting compounds. Protection/deprotection steps or time-consuming purification is not required, and the reactions can be scaled up to gram quantities. The procedures provide accessibility of these important compounds to atmospheric chemistry laboratories with only basic capabilities in organic synthesis.
Cited articles
Barnes, P. J. and Adcock, I. M.: NF-kB: a pivotal role in asthma and a new target for therapy, Am. J. Physiol, 265, 577–506, 1997.
Bowler, R. P. and Crapo, J. D.: Oxidative stress in allergic respiratory diseases, J. Allergy Clin. Immun., 110, 349–356, https://doi.org/10.1067/mai.2002.126780, 2002.
Budisulistiorini, S. H., Canagaratna, M. R., Croteau, P. L., Marth, W. J., Baumann, K., Edgerton, E. S., Shaw, S. L., Knipping, E. M., Worsnop, D. R., Jayne, J. T., Gold, A., and Surratt, J. D.: Real-Time Continuous Characterization of Secondary Organic Aerosol Derived from Isoprene Epoxydiols in Downtown Atlanta, Georgia, Using the Aerodyne Aerosol Chemical Speciation Monitor, Environ. Sci. Technol., 47, 5686–5694, https://doi.org/10.1021/es400023n, 2013.
Budisulistiorini, S. H., Li, X., Bairai, S. T., Renfro, J., Liu, Y., Liu, Y. J., McKinney, K. A., Martin, S. T., McNeill, V. F., Pye, H. O. T., Nenes, A., Neff, M. E., Stone, E. A., Mueller, S., Knote, C., Shaw, S. L., Zhang, Z., Gold, A., and Surratt, J. D.: Examining the effects of anthropogenic emissions on isoprene-derived secondary organic aerosol formation during the 2013 Southern Oxidant and Aerosol Study (SOAS) at the Look Rock, Tennessee ground site, Atmos. Chem. Phys., 15, 8871–8888, 10.5194/acp-15-8871-2015, 2015.
Budisulistiorini, S. H., Baumann, K., Edgerton, E. S., Bairai, S. T., Mueller, S., Shaw, S. L., Knipping, E. M., Gold, A., and Surratt, J. D.: Seasonal characterization of submicron aerosol chemical composition and organic aerosol sources in the southeastern United States: Atlanta, Georgia,and Look Rock, Tennessee, Atmos. Chem. Phys., 16, 5171–5189, https://doi.org/10.5194/acp-16-5171-2016, 2016.
Carlton, A. G., Wiedinmyer, C., and Kroll, J. H.: A review of Secondary Organic Aerosol (SOA) formation from isoprene, Atmos. Chem. Phys., 9, 4987–5005, https://doi.org/10.5194/acp-9-4987-2009, 2009.
de Bruijne, K., Ebersviller, S., Sexton, K. G., Lake, S., Leith, D., Goodman, R., Jetters, J., Walters, G. W., Doyle-Eisele, M., Woodside, R., Jeffries, H. E., and Jaspers, I.: Design and Testing of Electrostatic Aerosol In Vitro Exposure System (EAVES): An Alternative Exposure System for Particles, Inhal. Toxicol., 21, 91–101, https://doi.org/10.1080/08958370802166035, 2009.
Devlin, R. B., McKinnon, K. P., Noah, T., Becker, S., and Koren, H. S.: Ozone-induced release of cytokines and fibronectin by alveolar macrophages and airway epithelial cells, Am. J. Physiol.-Lung C., 266, L612–L619, 1994.
Dockery, D. W., Pope, C. A., Xu, X. P., Spengler, J. D., Ware, J. H., Fay, M. E., Ferris, B. G., and Speizer, F. E.: An Association between Air-Pollution and Mortality in 6 United-States Cities, New Engl. J. Med., 329, 1753–1759, 10.1056/NEJM199312093292401, 1993.
Doyle, M., Sexton, K. G., Jeffries, H., Bridge, K., and Jaspers, I.: Effects of 1,3-butadiene, isoprene, and their photochemical degradation products on human lung cells, Environ. Health Persp., 112, 1488–1495, https://doi.org/10.1289/ehp.7022, 2004.
Doyle, M., Sexton, K. G., Jeffries, H., and Jaspers, I.: Atmospheric photochemical transformations enhance 1,3-butadiene-induced inflammatory responses in human epithelial cells: The role of ozone and other photochemical degradation products, Chem.-Biol. Interact., 166, 163–169, https://doi.org/10.1016/j.cbi.2006.05.016, 2007.
Ebersviller, S., Lichtveld, K., Sexton, K. G., Zavala, J., Lin, Y.-H., Jaspers, I., and Jeffries, H. E.: Gaseous VOCs rapidly modify particulate matter and its biological effects – Part 1: Simple VOCs and model PM, Atmos. Chem. Phys., 12, 12277–12292, https://doi.org/10.5194/acp-12-12277-2012, 2012a.
Ebersviller, S., Lichtveld, K., Sexton, K. G., Zavala, J., Lin, Y.-H., Jaspers, I., and Jeffries, H. E.: Gaseous VOCs rapidly modify particulate matter and its biological effects – Part 2: Complex urban VOCs and model PM, Atmos. Chem. Phys., 12, 12293-12312, https://doi.org/10.5194/acp-12-12293-2012, 2012b.
FitzGerald, G. A.: COX-2 and beyond: approaches to prostaglandin inhibition in human disease, Nat. Rev. Drug. Discov., 2, 879–890, 2003.
Fong, C. Y., Pang, L., Holland, E., and Knox, A. J.: TGF-β1 stimulates IL-8 release, COX-2 expression, and PGE2release in human airway smooth muscle cells, Am. J. Physiol.-Lung C., 279, L201–L207, 2000.
Gangwal, S., Brown, J. S., Wang, A., Houck, K. A., Dix, D. J., Kavlock, R. J., and Hubal, E. A. C.: Informing Selection of Nanomaterial Concentrations for ToxCast in Vitro Testing Based on Occupational Exposure Potential, Environ. Health Persp., 119, 1539–1546, https://doi.org/10.1289/ehp.1103750, 2011.
Gaschen, A., Lang, D., Kalberer, M., Savi, M., Geiser, T., Gazdhar, A., Lehr, C.-M., Bur, M., Dommen, J., Baltensperger, U., and Geiser, M.: Cellular Responses after Exposure of Lung Cell Cultures to Secondary Organic Aerosol Particles, Environ. Sci. Technol., 44, 1424–1430, https://doi.org/10.1021/es902261m, 2010.
Gaston, C. J., Riedel, T. P., Zhang, Z. F., Gold, A., Surratt, J. D., and Thornton, J. A.: Reactive Uptake of an Isoprene-Derived Epoxydiol to Submicron Aerosol Particles, Environ. Sci. Technol., 48, 11178–11186, 10.1021/es5034266, 2014.
Hatch, G. E., Duncan, K. E., Diaz-Sanchez, D., Schmitt, M. T., Ghio, A. J., Carraway, M. S., McKee, J., Dailey, L. A., Berntsen, J., and Devlin, R. B.: Progress in Assessing Air Pollutant Risks from In Vitro Exposures: Matching Ozone Dose and Effect in Human Airway Cells, Toxicol. Sci., 141, 198–205, https://doi.org/10.1093/toxsci/kfu115, 2014.
Hawley, B. and Volckens, J.: Proinflammatory effects of cookstove emissions on human bronchial epithelial cells, Indoor Air, 23, 4–13, https://doi.org/10.1111/j.1600-0668.2012.00790.x, 2013.
Hawley, B., L'Orange, C., Olsen, D. B., Marchese, A. J., and Volckens, J.: Oxidative Stress and Aromatic Hydrocarbon Response of Human Bronchial Epithelial Cells Exposed to Petro- or Biodiesel Exhaust Treated with a Diesel Particulate Filter, Toxicol. Sci., 141, 505–514, https://doi.org/10.1093/toxsci/kfu147, 2014a.
Hawley, B., McKenna, D., Marchese, A., and Volckens, J.: Time course of bronchial cell inflammation following exposure to diesel particulate matter using a modified EAVES, Toxicol. in Vitro, 28, 829–837, https://doi.org/10.1016/j.tiv.2014.03.001, 2014b.
Hayashi, Y.: Designing in vitro assay systems for hazard characterization. Basic strategies and related technical issues, Exp. Toxicol. Pathol., 57, 227–232, https://doi.org/10.1016/j.etp.2005.05.012, 2005.
Henze, D. K., Seinfeld, J. H., Ng, N. L., Kroll, J. H., Fu, T.-M., Jacob, D. J., and Heald, C. L.: Global modeling of secondary organic aerosol formation from aromatic hydrocarbons: high- vs. low-yield pathways, Atmos. Chem. Phys., 8, 2405–2420, https://doi.org/10.5194/acp-8-2405-2008, 2008.
Hu, W. W., Campuzano-Jost, P., Palm, B. B., Day, D. A., Ortega, A. M., Hayes, P. L., Krechmer, J. E., Chen, Q., Kuwata, M., Liu, Y. J., de Sá, S. S., McKinney, K., Martin, S. T., Hu, M., Budisulistiorini, S. H., Riva, M., Surratt, J. D., St. Clair, J. M., Isaacman-Van Wertz, G., Yee, L. D., Goldstein, A. H., Carbone, S., Brito, J., Artaxo, P., de Gouw, J. A., Koss, A., Wisthaler, A., Mikoviny, T., Karl, T., Kaser, L., Jud, W., Hansel, A., Docherty, K. S., Alexander, M. L., Robinson, N. H., Coe, H., Allan, J. D., Canagaratna, M. R., Paulot, F., and Jimenez, J. L.: Characterization of a real-time tracer for isoprene epoxydiols-derived secondary organic aerosol (IEPOX-SOA) from aerosol mass spectrometer measurements, Atmos. Chem. Phys., 15, 11807–11833, https://doi.org/10.5194/acp-15-11807-2015, 2015.
Jang, M., Ghio, A. J., and Cao, G.: Exposure of BEAS-2B Cells to Secondary Organic Aerosol Coated on Magnetic Nanoparticles, Chem. Res. Toxicol., 19, 1044–1050, https://doi.org/10.1021/tx0503597, 2006.
Kelly, F. J. and Fussell, J. C.: Size, source and chemical composition as determinants of toxicity attributable to ambient particulate matter, Atmos. Environ., 60, 504–526, https://doi.org/10.1016/j.atmosenv.2012.06.039, 2012.
Kramer, A. J., Rattanavaraha, W., Zhang, Z., Gold, A., Surratt, J. D., and Lin, Y.-H.: Assessing the oxidative potential of isoprene-derived epoxides and secondary organic aerosol, Atmos. Environ., 130, 211–218, https://doi.org/10.1016/j.atmosenv.2015.10.018, 2016.
Krechmer, J. E., Coggon, M. M., Massoli, P., Nguyen, T. B., Crounse, J. D., Hu, W., Day, D. A., Tyndall, G. S., Henze, D. K., Rivera-Rios, J. C., Nowak, J. B., Kimmel, J. R., Mauldin, R. L., Stark, H., Jayne, J. T., Sipilä, M., Junninen, H., Clair, J. M. S., Zhang, X., Feiner, P. A., Zhang, L., Miller, D. O., Brune, W. H., Keutsch, F. N., Wennberg, P. O., Seinfeld, J. H., Worsnop, D. R., Jimenez, J. L., and Canagaratna, M. R.: Formation of Low Volatility Organic Compounds and Secondary Organic Aerosol from Isoprene Hydroxyhydroperoxide Low-NO Oxidation, Environ. Sci. Technol., 49, 10330–10339, https://doi.org/10.1021/acs.est.5b02031, 2015.
Kroll, J. H., Ng, N. L., Murphy, S. M., Flagan, R. C., and Seinfeld, J. H.: Secondary organic aerosol formation from isoprene photooxidation, Environ. Sci. Technol., 40, 1869–1877, https://doi.org/10.1021/es0524301, 2006.
Kunkel, S. L., Standiford, T., Kasahara, K., and Strieter, R. M.: Interleukin-8 (Il-8) – the Major Neutrophil Chemotactic Factor in the Lung, Exp. Lung Res., 17, 17–23, https://doi.org/10.3109/01902149109063278, 1991.
Li, H., Edin, M. L., Bradbury, J. A., Graves, J. P., DeGraff, L. M., Gruzdev, A., Cheng, J., Dackor, R. T., Wang, P. M., Bortner, C. D., Garantziotis, S., Jetten, A. M., and Zeldin, D. C.: Cyclooxygenase-2 Inhibits T Helper Cell Type 9 Differentiation during Allergic Lung Inflammation via Down-regulation of IL-17RB, Am. J. Resp. Crit. Care, 187, 812–822, https://doi.org/10.1164/rccm.201211-2073OC, 2013.
Li, N., Hao, M., Phalen, R. F., Hinds, W. C., and Nel, A. E.: Particulate air pollutants and asthma: A paradigm for the role of oxidative stress in PM-induced adverse health effects, Cl. Immunol., 109, 250–265, https://doi.org/10.1016/j.clim.2003.08.006, 2003.
Lichtveld, K. M., Ebersviller, S. M., Sexton, K. G., Vizuete, W., Jaspers, I., and Jeffries, H. E.: In Vitro Exposures in Diesel Exhaust Atmospheres: Resuspension of PM from Filters versus Direct Deposition of PM from Air, Environ. Sci. Technol., 46, 9062–9070, https://doi.org/10.1021/es301431s, 2012.
Lin, Y.-H., Zhang, Z., Docherty, K. S., Zhang, H., Budisulistiorini, S. H., Rubitschun, C. L., Shaw, S. L., Knipping, E. M., Edgerton, E. S., Kleindienst, T. E., Gold, A., and Surratt, J. D.: Isoprene Epoxydiols as Precursors to Secondary Organic Aerosol Formation: Acid-Catalyzed Reactive Uptake Studies with Authentic Compounds, Environ. Sci. Technol., 46, 250–258, https://doi.org/10.1021/es202554c, 2012.
Lin, Y.-H., Knipping, E. M., Edgerton, E. S., Shaw, S. L., and Surratt, J. D.: Investigating the influences of SO2 and NH3 levels on isoprene-derived secondary organic aerosol formation using conditional sampling approaches, Atmos. Chem. Phys., 13, 8457–8470, https://doi.org/10.5194/acp-13-8457-2013, 2013a.
Lin, Y.-H., Zhang, H., Pye, H. O. T., Zhang, Z., Marth, W. J., Park, S., Arashiro, M., Cui, T., Budisulistiorini, S. H., Sexton, K. G., Vizuete, W., Xie, Y., Luecken, D. J., Piletic, I. R., Edney, E. O., Bartolotti, L. J., Gold, A., and Surratt, J. D.: Epoxide as a precursor to secondary organic aerosol formation from isoprene photooxidation in the presence of nitrogen oxides, P. Natl. Acad. Sci. USA, 110, 6718–6723, https://doi.org/10.1073/pnas.1221150110, 2013b.
Lin, Y.-H., Arashiro, M., Martin, E., Chen, Y., Zhang, Z., Sexton, K. G., Gold, A., Jaspers, I., Fry, R. C., and Surratt, J. D.: Isoprene-Derived Secondary Organic Aerosol Induces the Expression of Oxidative Stress Response Genes in Human Lung Cells, Environ. Sci. Technol. Lett., 3, 250–254, https://doi.org/10.1021/acs.estlett.6b00151, 2016.
Liu, J., D'Ambro, E. L., Lee, B. H., Lopez-Hilfiker, F. D., Zaveri, R. A., Rivera-Rios, J. C., Keutsch, F. N., Iyer, S., Kurten, T., Zhang, Z., Gold, A., Surratt, J. D., Shilling, J. E., and Thornton, J. A.: Efficient Isoprene Secondary Organic Aerosol Formation from a Non-IEPOX Pathway, Environ. Sci. Technol., 50, 9872–9880, 2016.
Livak, K. J. and Schmittgen, T. D.: Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2–ΔΔCT Method, Methods, 25, 402–408, https://doi.org/10.1006/meth.2001.1262, 2001.
Nguyen, T. B., Bates, K. H., Crounse, J. D., Schwantes, R. H., Zhang, X., Kjaergaard, H. G., Surratt, J. D., Lin, P., Laskin, A., and Seinfeld, J. H.: Mechanism of the hydroxyl radical oxidation of methacryloyl peroxynitrate (MPAN) and its pathway toward secondary organic aerosol formation in the atmosphere, Phys. Chem. Chem. Phys., 17, 17914–17926, 2015.
Nocker, R. E. T., Schoonbrood, D. F. M., vandeGraaf, E. A., Hack, C. E., Lutter, R., Jansen, H. M., and Out, T. A.: Interleukin-8 in airway inflammation in patients with asthma and chronic obstructive pulmonary disease, Int. Arch. Allergy Imm., 109, 183–191, 1996.
Paulot, F., Crounse, J. D., Kjaergaard, H. G., Kuerten, A., St Clair, J. M., Seinfeld, J. H., and Wennberg, P. O.: Unexpected Epoxide Formation in the Gas-Phase Photooxidation of Isoprene, Science, 325, 730–733, https://doi.org/10.1126/science.1172910, 2009.
Paur, H.-R., Cassee, F. R., Teeguarden, J., Fissan, H., Diabate, S., Aufderheide, M., Kreyling, W. G., Hänninen, O., Kasper, G., Riediker, M., Rothen-Rutishauser, B., and Schmid, O.: In-vitro cell exposure studies for the assessment of nanoparticle toxicity in the lung – A dialog between aerosol science and biology, J. Aerosol Sci., 42, 668–692, https://doi.org/10.1016/j.jaerosci.2011.06.005, 2011.
Peng, H., Chen, P., Cai, Y., Chen, Y., Wu, Q.-H., Li, Y., Zhou, R., and Fang, X.: Endothelin-1 increases expression of cyclooxygenase-2 and production of interlukin-8 in hunan pulmonary epithelial cells, Peptides, 29, 419–424, https://doi.org/10.1016/j.peptides.2007.11.015, 2008.
Pye, H. O. T., Pinder, R. W., Piletic, I. R., Xie, Y., Capps, S. L., Lin, Y.-H., Surratt, J. D., Zhang, Z., Gold, A., Luecken, D. J., Hutzell, W. T., Jaoui, M., Offenberg, J. H., Kleindienst, T. E., Lewandowski, M., and Edney, E. O.: Epoxide Pathways Improve Model Predictions of Isoprene Markers and Reveal Key Role of Acidity in Aerosol Formation, Environ. Sci. Technol., 47, 11056–11064, https://doi.org/10.1021/es402106h, 2013.
Rager, J. E., Lichtveld, K., Ebersviller, S., Smeester, L., Jaspers, I., Sexton, K. G., and Fry, R. C.: A Toxicogenomic Comparison of Primary and Photochemically Altered Air Pollutant Mixtures, Environ. Health Persp., 119, 1583–1589, https://doi.org/10.1289/ehp.1003323, 2011.
Rattanavaraha, W., Chu, K., Budisulistiorini, S. H., Riva, M., Lin, Y.-H., Edgerton, E. S., Baumann, K., Shaw, S. L., Guo, H., King, L., Weber, R. J., Neff, M. E., Stone, E. A., Offenberg, J. H., Zhang, Z., Gold, A., and Surratt, J. D.: Assessing the impact of anthropogenic pollution on isoprene-derived secondary organic aerosol formation in PM2.5 collected from the Birmingham, Alabama, ground site during the 2013 Southern Oxidant and Aerosol Study, Atmos. Chem. Phys., 16, 4897–4914, https://doi.org/10.5194/acp-16-4897-2016, 2016.
Reddel, R. R., Ke, Y., Gerwin, B. I., McMenamin, M. G., Lechner, J. F., Su, R. T., Brash, D. E., Park, J.-B., Rhim, J. S., and Harris, C. C.: Transformation of Human Bronchial Epithelial Cells by Infection with SV40 or Adenovirus-12 SV40 Hybrid Virus, or Transfection via Strontium Phosphate Coprecipitation with a Plasmid Containing SV40 Early Region Genes, Cancer Res., 48, 1904–1909, 1988.
Reuter, S., Gupta, S. C., Chaturvedi, M. M., and Aggarwal, B. B.: Oxidative stress, inflammation, and cancer: How are they linked?, Free Radical Bio. Med., 49, 1603–1616, https://doi.org/10.1016/j.freeradbiomed.2010.09.006, 2010.
Riedel, T. P., Lin, Y.-H., Budisulistiorini, S. H., Gaston, C. J., Thornton, J. A., Zhang, Z., Vizuete, W., Gold, A., and Surratt, J. D.: Heterogeneous Reactions of Isoprene-Derived Epoxides: Reaction Probabilities and Molar Secondary Organic Aerosol Yield Estimates, Environ. Sci. Technol. Lett., 2, 38–42, https://doi.org/10.1021/ez500406f, 2015.
Riedel, T. P., Lin, Y.-H., Zhang, Z., Chu, K., Thornton, J. A., Vizuete, W., Gold, A., and Surratt, J. D.: Constraining condensed-phase formation kinetics of secondary organic aerosol components from isoprene epoxydiols, Atmos. Chem. Phys., 16, 1245–1254, https://doi.org/10.5194/acp-16-1245-2016, 2016.
Riva, M., Budisulistiorini, S. H., Chen, Y., Zhang, Z., D'Ambro, E. L., Zhang, X., Gold, A., Turpin, B. J., Thornton, J. A., Canagaratna, M. R., and Surratt, J. D.: Chemical Characterization of Secondary Organic Aerosol from Oxidation of Isoprene Hydroxyhydroperoxides, Environ. Sci. Technol., 50, 9889–9899, 2016.
Rohr, A. C.: The health significance of gas- and particle-phase terpene oxidation products: A review, Environ. Int., 60, 145–162, https://doi.org/10.1016/j.envint.2013.08.002, 2013.
Rohr, A. C., Shore, S. A., and Spengler, J. D.: Repeated exposure to isoprene oxidation products causes enhanced respiratory tract effects in multiple murine strains, Inhal. Toxicol., 15, 1191–1207, https://doi.org/10.1080/08958370390229870, 2003.
Samet, J. M., Dominici, F., Curriero, F. C., Coursac, I., and Zeger, S. L.: Fine particulate air pollution and mortality in 20 US Cities, 1987–1994, New England Journal of Medicine, 343, 1742–1749, https://doi.org/10.1056/NEJM200012143432401, 2000.
Schreck, R., Albermann, K., and Baeuerle, P. A.: Nuclear Factor Kb: An Oxidative Stress-Responsive Transcription Factor of Eukaryotic Cells (A Review), Free Radical Res. Comm., 17, 221–237, https://doi.org/10.3109/10715769209079515, 1992.
Schwartz, J., Slater, D., Larson, T. V., Pierson, W. E., and Koenig, J. Q.: Particulate Air-Pollution and Hospital Emergency Room Visits for Asthma in Seattle, Am. Rev. Respir. Dis., 147, 826–831, 1993.
Seagrave, J.: Mechanisms and implications of air pollution particle associations with chemokines, Toxicol. Appl. Pharm., 232, 469–477, https://doi.org/10.1016/j.taap.2008.08.001, 2008.
Sies, H.: Oxidants And Antioxidants: Pathophysiologic Determinants and Therapeutic AgentsOxidative stress: From basic research to clinical application, Am. J. Med., 91, S31–S38, https://doi.org/10.1016/0002-9343(91)90281-2, 1991.
Surratt, J. D., Murphy, S. M., Kroll, J. H., Ng, N. L., Hildebrandt, L., Sorooshian, A., Szmigielski, R., Vermeylen, R., Maenhaut, W., and Claeys, M.: Chemical composition of secondary organic aerosol formed from the photooxidation of isoprene, J. Phys. Chem. A, 110, 9665–9690, 2006.
Surratt, J. D., Kroll, J. H., Kleindienst, T. E., Edney, E. O., Claeys, M., Sorooshian, A., Ng, N. L., Offenberg, J. H., Lewandowski, M., Jaoui, M., Flagan, R. C., and Seinfeld, J. H.: Evidence for organosulfates in secondary organic aerosol, Environ. Sci. Technol., 41, 517–527, https://doi.org/10.1021/es062081q, 2007.
Surratt, J. D., Chan, A. W. H., Eddingsaas, N. C., Chan, M., Loza, C. L., Kwan, A. J., Hersey, S. P., Flagan, R. C., Wennberg, P. O., and Seinfeld, J. H.: Reactive intermediates revealed in secondary organic aerosol formation from isoprene, P. Natl. Acad Sci. USA, 107, 6640–6645, https://doi.org/10.1073/pnas.0911114107, 2010.
Tao, F., Gonzalez-Flecha, B., and Kobzik, L.: Reactive oxygen species in pulmonary inflammation by ambient particulates, Free Radical Bio. Med., 35, 327–340, https://doi.org/10.1016/S0891-5849(03)00280-6, 2003.
Uchida, K.: A Lipid-derived Endogenous Inducer of COX-2: a Bridge Between Inflammation and Oxidative Stress, Mol. Cells, 25, 347–351, 2008.
Volckens, J., Dailey, L., Walters, G., and Devlin, R. B.: Direct Particle-to-Cell Deposition of Coarse Ambient Particulate Matter Increases the Production of Inflammatory Mediators from Cultured Human Airway Epithelial Cells, Environ. Sci. Technol., 43, 4595–4599, https://doi.org/10.1021/es900698a, 2009.
Xu, L., Guo, H. Y., Boyd, C. M., Klein, M., Bougiatioti, A., Cerully, K. M., Hite, J. R., Isaacman-VanWertz, G., Kreisberg, N. M., Knote, C., Olson, K., Koss, A., Goldstein, A. H., Hering, S. V., de Gouw, J., Baumann, K., Lee, S. H., Nenes, A., Weber, R. J., and Ng, N. L.: Effects of anthropogenic emissions on aerosol formation from isoprene and monoterpenes in the southeastern United States, P. Natl. Acad. Sci. USA, 112, 37–42, https://doi.org/10.1073/pnas.1417609112, 2015.
Yan, Z., Wang, J., Li, J., Jiang, N., Zhang, R., Yang, W., Yao, W., and Wu, W.: Oxidative stress and endocytosis are involved in upregulation of interleukin-8 expression in airway cells exposed to PM2.5, Environ. Toxicol., https://doi.org/10.1002/tox.22188, online first, 2015.
Zavala, J., Lichtveld, K., Ebersviller, S., Carson, J. L., Walters, G. W., Jaspers, I., Jeffries, H. E., Sexton, K. G., and Vizuete, W.: The Gillings Sampler – An electrostatic air sampler as an alternative method for aerosol in vitro exposure studies, Chem.-Biol. Interact., 220, 158–168, https://doi.org/10.1016/j.cbi.2014.06.026, 2014.
Zhang, Z., Lin, Y.-H., Zhang, H., Surratt, J. D., Ball, L. M., and Gold, A.: Technical Note: Synthesis of isoprene atmospheric oxidation products: isomeric epoxydiols and the rearrangement products cis- and trans-3-methyl-3,4-dihydroxytetrahydrofuran, Atmos. Chem. Phys., 12, 8529–8535, https://doi.org/10.5194/acp-12-8529-2012, 2012.
Short summary
Atmospheric oxidation of isoprene in the presence of acidic sulfate aerosol yields substantial SOA. Potential adverse health effects resulting from exposure to this aerosol type are largely unknown. Measurements of gene expression of known inflammatory biomarkers interleukin 8 (IL-8) and cyclooxygenase 2 (COX-2) in exposed human lung cells at the air–liquid interface showed that a dose of 0.067 μg cm−2 of isoprene SOA leads to statistically significant increases in IL-8 and COX-2 mRNA levels.
Atmospheric oxidation of isoprene in the presence of acidic sulfate aerosol yields substantial...
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