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Atmospheric Chemistry and Physics An interactive open-access journal of the European Geosciences Union
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ACP | Articles | Volume 20, issue 8
Atmos. Chem. Phys., 20, 4593–4605, 2020
https://doi.org/10.5194/acp-20-4593-2020
© Author(s) 2020. This work is distributed under
the Creative Commons Attribution 4.0 License.

Special issue: Multiphase chemistry of secondary aerosol formation under...

Atmos. Chem. Phys., 20, 4593–4605, 2020
https://doi.org/10.5194/acp-20-4593-2020
© Author(s) 2020. This work is distributed under
the Creative Commons Attribution 4.0 License.

Research article 21 Apr 2020

Research article | 21 Apr 2020

The evolutionary behavior of chromophoric brown carbon during ozone aging of fine particles from biomass burning

The evolutionary behavior of chromophoric brown carbon during ozone aging of fine particles from biomass burning
Xingjun Fan1,3, Tao Cao2,4, Xufang Yu1, Yan Wang1, Xin Xiao1, Feiyue Li1,3, Yue Xie1, Wenchao Ji1, Jianzhong Song2, and Ping'an Peng2 Xingjun Fan et al.
  • 1College of Resource and Environment, Anhui Science and Technology University, Fengyang 233100, P. R. China
  • 2State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, P. R. China
  • 3Anhui Province Key Laboratory of Biochar and Cropland Pollution Prevention, Bengbu 233400, P. R. China
  • 4University of Chinese Academy of Sciences, Beijing 100049, P. R. China

Correspondence: Jianzhong Song (songjzh@gig.ac.cn) and Xingjun Fan (fanxj@ahstu.edu.cn)

Abstract
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Biomass burning (BB) emits large amounts of brown carbon (BrC); however, the evolutionary behavior of BrC in BB emissions (BB BrC) resulting from complex atmospheric processes is poorly understood. In this study, the transformation of contents and the chromophoric characteristics of BrC in smoke particles emitted by the burning of rice straw (RS), corn straw (CS), and pinewood (PW) under O3 aging are investigated. The O3 aging induced the reduction of light absorption and fluorescence for the BB BrC, suggesting the decomposition of chromophores and fluorophores. These changes were accompanied by a decrease in aromaticity, average molecular weight, and the light absorption capacity for the chromophores, as well as an increase in humification for the fluorophores. The excitation emission matrix combined with a parallel factor analysis revealed that protein-like components (C3) were predominantly decomposed by O3 aging, while the relative distribution of a humic-like component with highly oxygenated chromophores (C4) gradually increased. In general, the humic-like substances (C1 + C2 + C4) were transformed to be the most abundant fluorophores for all the BB BrC samples, which accounted for 84 %–87 % of the total fluorophores in final O3-aged BB BrC. Two-dimensional correlation spectroscopy (2D-COS) was performed on the synchronous fluorescence, which suggested that the RS and CS BrC exhibits the same susceptible fluorophores changes upon O3 aging. It showed that O3 firstly reacted with protein-like fractions (263–289 nm) and then with fulvic-like fractions (333–340 nm). In comparison, the changing sequence of susceptible fluorophores in the PW BrC to O3 was in the order of fulvic-like fluorophores with shorter wavelengths (309 nm), protein-like fluorophores (276 nm), and fulvic-like fluorophores with longer wavelengths (358 nm). The 2D-FTIR-COS (2D-COS combined with FTIR) analysis showed conjugated C=O and aromatic C=C and C=O groups were the most susceptible functional groups to O3 aging for all BB BrC. Moreover, it also revealed a consistent sequential change, which is in the order of aromatic OH; conjugated C=O groups and aromatic C=O; aromatic COO; and finally lignin-derived C–C, C–H, and C–O groups. Our results provide new insights into the evolutionary behavior of the chromophoric and fluorescent properties of BB BrC during O3 aging, which are of great significance for better understanding the heterogeneous oxidation pathways of BB-derived BrC in the atmospheric environment.

1 Introduction
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Brown carbon (BrC), as a fraction of organic aerosols with effective light absorption properties, has been a research hotspot in the atmospheric carbonaceous aerosols field (Andreae and Gelencser, 2006; Laskin et al., 2015; Yan et al., 2018). BrC strongly absorbs solar radiation at short ultraviolet–visible wavelengths, which has been estimated to contribute 6 %–28 % of the total atmospheric aerosol absorption (Chen et al., 2018; Chung et al., 2012; Feng et al., 2013; Liu et al., 2015). Owing to its significant effect on light absorption, BrC can affect both direct radiative forcing and atmospheric photochemistry (Mok et al., 2016; Jo et al., 2016). In addition, BrC may have an adverse effect on human health due to its capacity of enhancing the oxidative potential of atmospheric aerosols (Chen et al., 2019; Ma et al., 2018).

Atmospheric BrC arises from multiple sources, including biomass burning (BB), coal combustion, and secondary atmospheric chemical reactions (Bond, 2004; Chen et al., 2018; Fan et al., 2018; Hecobian et al., 2010; Saleh et al., 2013; Wang et al., 2018). Among them, BB is believed to be one of the important sources (Chen and Bond, 2010; Fan et al., 2016, 2018; Huo et al., 2018; Park and Yu, 2016). Recently, primary BB BrC has been widely studied, which has provided deep insights into its BB source-dependent chemical and optical properties (Fan et al., 2016, 2018; Park and Yu, 2016). Moreover, both laboratory and field studies have demonstrated that atmospheric aging processes will lead to a transformation of the chemical composition and optical properties of BB BrC once it is emitted into the atmosphere (Fan et al., 2019; Pillar et al., 2014, 2015, 2017; Saleh et al., 2013; Schnitzler and Abbatt, 2018; Sumlin et al., 2017; Wong et al., 2017, 2019; Zhong and Jang, 2014). The relevant processes mainly occur in either the atmospheric aqueous phase (Fan et al., 2019; Wong et al., 2017, 2019; Zhao et al., 2015), gas phase (Schnitzler and Abbatt, 2018; Sumlin et al., 2017; Zhong and Jang, 2014), or air–particle interface (Pillar-Little and Guzma, 2018, and references therein) with homogeneous or heterogeneous oxidation.

Most studies have investigated BB BrC aging processes with a focus on aqueous-phase oxidations, such as photochemistry (Chang and Thompson, 2010; Zhao et al., 2015), dark OH-induced oxidation (G. T. Santos et al., 2016; P. S. Santos et al., 2016; Santos and Duarte, 2015), and carbonyl compound reactions with amines/ammonium sulfate (AS) (De Haan et al., 2017; Tang et al., 2016). The oxidation mechanisms and evolution of the optical properties of individual water-soluble organic compounds derived from BB emissions, such as levoglucosan (Holmes and Petrucci, 2006), aromatic acids (P. S. Santos et al., 2016), phenolic compounds (Lavi et al., 2017), nitrophenols (Hems and Abbatt, 2018; Lin et al., 2015), methylglyoxal (De Haan et al., 2017), and some other unsaturated carboxylic acids (Gallimore et al., 2011; Lee and Chan, 2007), have been well illustrated. The results suggest that the chromophoric characteristics of aged BrC are highly variable across different precursors and oxidation conditions. Many recent laboratory simulation studies have also tentatively explored the evolution and oxidation mechanisms of water-soluble BB BrC in the aqueous phase with or without light (Fan et al., 2019; Wong et al., 2017, 2019). These studies have observed that BB BrC undergoes both enhancement and bleaching, but a significant bleaching of BrC dominantly occurs over a long aging time. However, these results are far from definitively pronouncing the evolutionary behavior of BB BrC, mainly because of the high complexity of BB emission (i.e., nonpolar and polar organic compounds, soot particles) and atmospheric oxidants (i.e., O3, NO2, OH radicals).

The heterogeneous oxidations are also important pathways to form or bleach BrC, which have been widely studied for the aging of soot and individual organic compounds by OH, O3, NOx, SO2, and HONO (Baduel et al., 2011; Gallimore et al., 2011; He et al., 2017; Li et al., 2013, 2015, 2017; Pillar-Little and Guzman, 2018, and references therein). Among them, the O3 aging simulations have been widely conducted, mainly because of O3 is not only a significant pollutant but also an important oxidant in the atmosphere (Chapleski et al., 2016; Chi et al., 2018; Li et al., 2018). The O3 oxidation of soot particles generally changes their physicochemical properties (i.e., water solubility, hygroscopicity, size, oxidative potential, and morphology), as well as forms new chromophoric compounds (Decesari et al., 2002; Li et al., 2013; Zhu et al., 2019). In addition, the O3 oxidation of organic compounds, such as humic-like substances (HULISs), catechol, and its substituted ones, commonly leads to competitive fragmentation and functionalization of chromophores (Baduel et al., 2011; D'Anna et al., 2009; Pillar et al., 2014, 2015, 2017; Sun et al., 2019). To date, however, there has been no systematic investigation on the evolutionary behavior of bulk BrC, especially in terms of its chromophoric characteristics, via heterogeneous O3 oxidation of complex BB emissions.

The objective of this study is to comprehensively investigate the evolutionary behavior of fine particulate chromophoric BrC produced by BB during the O3 aging process. For this purpose, the O3 aging of three types of BB smoke particles emitted from rice straw (RS), corn straw (CS), and pinewood (PW) is conducted in a reactor. Then, the levels and chromophoric characteristics of fresh and aged BrC are determined with a total organic carbon (TOC) analysis, ultraviolet–visible (UV–Vis) spectroscopy, an excitation-emission matrix (EEM) fluorescence spectroscopy, and Fourier transform infrared (FTIR) spectroscopy. Furthermore, the O3 aging mechanism of BB BrC is explored using EEM combined with a parallel factor analysis (PARAFAC) and a two-dimensional correlation analysis (2D-COS) applied on the synchronous fluorescence spectra and FTIR. These approaches have allowed us to investigate the levels and types of independent underlying fluorophores of BrC (Chen et al., 2016b; Fan et al., 2019) and explore the dynamic spectral behavior of chromophores and molecular structures of BrC (Fan et al., 2019; Duarte et al., 2015). Our results have great importance in terms of furthering source apportionment of atmospheric BrC and of improving the accuracy of predicting their effects in atmospheric environments and on climate change.

2 Experimental section
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2.1 Preparation of the BB smoke particles

The BB smoke particles were prepared in a laboratory sampling system, which was described in detail in our previous study (Fan et al., 2018). The burning experiments were conducted without any control conditions, which is most similar to a natural BB process. Briefly, small pieces of RS, CS, and PW biomass materials were placed on stainless-steel mesh in a combustor and then ignited with an electronic gas lighter. The resulting smoke was introduced into a mixing chamber with diluted air, and the fine smoke particles were collected on quartz filters (Whatman, Ø 90 mm) using a PM2.5 sampler (Tianhong Intelligent Instrument Plant, Wuhan, China) at a flow rate of 80 L min−1. Finally, BB particles were obtained from each of the three fuels.

2.2 Ozone aging of BB smoke particles

The O3 aging experiments of the BB smoke samples were conducted in a self-designed reactor. A schematic diagram of the ozone aging reactor is illustrated in Fig. 1. The construction of the reactor and its operation are described in detail in Sect. S1 of the Supplement. For O3 aging, the sampled BB smoke particle filters were first cut into pieces (area of each piece is equal to 1.5 cm2) and then uniformly spread in a glass dish (Φ=90 mm) before being exposed in an O3 environment in the principal reactor. During the aging process, the temperature, relative humidity (RH), and O3 concentration in the principal reactor were maintained at 25 C, 40 % and 70 ppm, respectively. To avoid any photolysis of O3 and any photochemical reactions, all components in the reactor were wrapped with aluminum foil. Additionally, control experiments were conducted, but no variations of the chromophoric BrC were observed when there was no ozone introduced (Sect. S2, Figs. S1–S2 in the Supplement).

It should be noted that some gas-phase artifacts (i.e., semivolatile and intermediate-volatility organic compounds) possibly adsorbed on the quartz filters during sampling (Geller et al., 2006; Parshintsev et al., 2011). However, the amounts of these adsorbed organic artifacts on the filters are very small (0.48–0.98 µgC cm−2) (Arhami et al., 2006; Subramanian et al., 2004) and are much lower than the amounts of the OC fraction (∼250–750 µgC cm−2) in fresh BB smoke samples. Therefore, the potential contributions from O3 oxidation of gas-phase artifacts on filters to BB BrC can be neglected.

https://www.atmos-chem-phys.net/20/4593/2020/acp-20-4593-2020-f01

Figure 1The experimental apparatus.

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2.3 Water-soluble BrC extraction

At each designed exposure time (0, 1, 2, 4, 8, 12, and 24 h), three pieces of smoke filter for each BB sample were taken out and immersed in ultrapure water. After extracting with sonication for 30 min, the extracts were filtered through a 0.22 µm polytetrafluoroethylene syringe filter (Jinteng, Tianjin, China). The filtrates obtained were designated as water-soluble BrC.

2.4 Analytical techniques

The organic carbon (OC) content of the fresh and aged water-soluble BB BrC samples was measured using a total organic carbon analyzer (TOC-VCPN, Shimadzu, Kyoto, Japan). The UV–Vis absorption spectra were obtained using a spectrophotometer (UV-2600, Shimadzu, Kyoto, Japan) at wavelengths ranging from 200 to 700 nm. The synchronous fluorescence (SF) spectra were measured using a F-4600 fluorescence spectrometer (Hitachi, Tokyo, Japan) at excitation wavelengths ranging from 200 to 400 nm with a constant offset (Δλ=60 nm). The EEM spectra were also recorded using the F-4600 fluorescence spectrometer, and the scanning ranges were set to 220–400 nm for excitation and 290–520 nm for emission, with a 5 nm interval. The scanning speed was set to 12 000 nm min−1. Ultrapure water was used as blanks to correct the sample UV–Vis, SF, and EEM spectra. In addition, portions of the sample solutions were freeze-dried before the FTIR analysis. The FTIR spectra (4000–400 cm−1) were recorded at room temperature using a Nicolet iS10 FTIR spectrometer (Thermo, USA), and each spectrum was obtained after 64 scans with 4 cm−1 resolution. To describe the aging behavior of the BB BrC, some useful optical parameters, including the specific UV absorbance at 254 nm (SUVA254) (Fan et al., 2016), the absorption Ångström exponent (AAE) (Fan et al., 2016; Cheng et al., 2016; Huo et al., 2018; Park and Yu, 2016), mass absorption efficiencies (MAE365) (Chen et al., 2016a; Fan et al., 2016; Huo et al., 2018; Park and Yu, 2016), and the humification index (HIX) (Qin et al., 2018), were comparatively analyzed. Their calculation protocols are described in detail in Sect. S3.

2.5 Parallel factor analysis

PARAFAC was performed using MATLAB R2014a (MathWorks, USA) with the DOMFluor toolbox following the procedure introduced by Stedmon and Bro (2008). PARAFAC was computed using two- to seven-component models with nonnegativity constraints, and a four-component model was split-half validated on both halves using 63 distinct EEMs for all BB BrC samples. It is noted that all EEMs were normalized to the area under the Milli-Q water Raman peak (Ex = 350 nm, Em = 365–430 nm), which was collected on the same day to produce corrected fluorescence intensities in Raman units (Lawaetz and Stedmon, 2009). Furthermore, the relative levels of the individual fluorophores were evaluated using the corresponding maximum fluorescence intensity (Fmax) (Zhang et al., 2014; Matos et al., 2015).

2.6 2D-COS

The SF spectra (200–400 nm) and FTIR spectra (1800–1000 cm−1) of each type of BrC were analyzed using 2D-COS, with the oxidation time as the perturbation variable. The calculations were performed using the 2D Shige software (Shigeaki Morita, Kwansei Gakuin University, Japan, 2004–2005). Details of the algorithm adopted in the software can be found in previous studies (Duarte et al., 2015; Jin et al., 2018; Hur and Lee, 2014). Finally, the synchronous and asynchronous maps were plotted by using the OriginPro 2018 software (OriginLab, USA).

3 Results and discussion
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3.1 Changes in water-soluble BrC levels in BB smoke particles

Changes in the water-soluble organic carbon (WSOC) content of BrC, calculated as WSOC[t]/WSOC[0], for three types of BB smoke particles during the O3 aging process are shown in Fig. 2a. An initial rapid increase (0–2 h) and a final slight decrease (8–24 h) in WSOC for the three types of aged BB-produced smoke particles can be observed. However, some apparent differences were also identified for the three BB samples from 2 to 8 h. During this period, the WSOC[t]/WSOC[0] values first decreased from 1.05 and 1.07 after 2 h to 0.99 and 1.04 after 4 h and then increased to 1.06 and 1.09 after 8 h for the RS and CS samples, respectively. In comparison, the WSOC[t]/WSOC[0] values increased from 1.16 after 2 h to 1.22 after 4 h and then decreased to 1.18 after 8 h for the PW samples.

According to previous studies, the O3 oxidation of soot particles could produce some new WSOC fractions (Decesari et al., 2002; Li et al., 2015), and O3 may also destroy the C=C bonds within weakly and even nonpolar organic compounds to generate newly highly hydrophilic O-containing functional components mainly through oxygen addition (i.e., carboxyl, hydroxyl, carbonyl, and phenol-OH groups) (Baduel et al., 2011). On the other hand, O3 could lead to C–C bond cleavage within OC fractions to produce many volatile species, such as volatile organic compounds, CO, and CO2, resulting in a loss of carbon mass (D'Anna et al., 2009). Due to the complex composition of BB emissions, the formation and decomposition of WSOC simultaneously occurs, and its composition dynamically changes. The initial rapid increase in WSOC content indicates that the formation of WSOC from the oxidation of the soot particles is likely the dominant reaction, and the observable decreasing trends of WSOC content might indicate that the amount being formed is unable to compensate for the amount that decomposes.

https://www.atmos-chem-phys.net/20/4593/2020/acp-20-4593-2020-f02

Figure 2Dynamic variations of (a) WSOC content and (b) light absorption at a wavelength of 365 nm by the BB BrC during the ozone aging process.

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Changes in the total absorption at 365 nm (abs365) were investigated to illustrate the evolution of the chromophores in BB BrC during the O3 aging process (Fig. 2b). The abs365 values of BrC for the three BB smoke samples all gradually decreased with an increase in the aging time. As shown in Fig. 2b, a 41 %–49 % decrease in abs365 was seen for 24 h of O3 aging, indicating that the BB BrC underwent significant bleaching. Given that some increase in the WSOC content was observed during the O3 aging process, the decreased abs365 may have been driven by a loss of highly absorbent WSOC and/or the formation of nonabsorptive/weakly absorptive WSOC fractions (Eugene et al., 2016; Lavi et al., 2017; Pillar et al., 2014, 2015; Rincón et al., 2009, 2010; Xia et al., 2018). This was consistent with the previous findings that the bleaching of BB-derived chromophores, such as oxocarboxylic acids and oxy-aromatics, generally resulted in formation of non-light-absorbing compounds, such as aliphatic mono- or polycarboxylic acids, containing alcohol –OH, aldehyde –C(H)=O, and ether –O– groups (Eugene et al., 2016; Lavi et al., 2017; Pillar et al., 2014, 2015; Rincón et al., 2009, 2010; Xia et al., 2018). Moreover, the changes in abs365 for the three types of BB BrC also exhibited some differences. The PW BrC presented higher degrees of bleaching than the CS and RS BrC (Fig. 2b). This suggests that the bleaching of chromophoric BrC within PW smoke particles during O3 aging is much more likely to occur than for CS and RS smoke particles.

3.2 Variations of the chromophoric characteristics of BrC

In the present study, SUVA254, HIX, MAE365, and AAE were investigated to characterize the evolution of the aromaticity, humification degree, and light-absorbing properties of BB BrC during O3 aging. As shown in Fig. 3a, the BB BrC SUVA254 values gradually declined, especially during the first hour, implying a significant decrease in aromaticity during O3 aging. This may be attributed to the decomposition of aromatic species and/or the formation of many more hydrophilic organic compounds with weak-light-absorption or even non-light-absorption properties. The finding agreed well with the observations that the O3 oxidation of BB-derived chromophores (i.e., oxy-aromatics) could lead to cleavage of aromatic bonds into polyfunctional low-molecular-weight carboxylic acids (Pillar et al., 2014, 2015, 2017). The noticeable HIX increases seen for the three types of BB BrC indicate that the O3 aging may strongly decompose the protein-like fluorophores, probably phenolic compounds (Chen et al., 2016a), to form polyhydroxylated aromatic species or newly humic-like fluorophores (Pillar et al., 2014, 2015, 2017; Decesari et al., 2002; Li et al., 2013) (Fig. 3b). For example, O3 oxidation of phenolic compounds could form polyhydroxylated aromatic compounds with absorption redshift, which might lead to their HIX values increasing (Lavi et al., 2017; Magalhães et al., 2017; Pillar et al., 2015; Rincón et al., 2009, 2010). There seems to be contradictions between the results of the SUVA254 and HIX analyses, but together they are reasonable. It is known that SUVA254 reflects the average aromaticity of entire WSOC fractions (Fan et al., 2016, 2018), which can be greatly reduced by weakly/non-light-absorbing organic compounds formed during O3 aging. However, HIX represents the humification characteristics of fluorophores within aged BrC (Qin et al., 2018), of which the influence of nonchromophores is excluded.

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Figure 3Dynamic variations of (a) SUVA254, (b) HIX, (c) MAE365, and (d) AAE of RS, CS, and PW smoke water-soluble BrC during the ozone aging process.

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The MAE365 values of the BB BrC showed a gradual decline as a function of oxidation time (Fig. 3c), giving rise to the MAE365 values of the 24 h aged BB BrC being reduced by a factor of 1.6–2.2. This suggests that the absorption efficiency of BrC was weakened by the O3 aging, which greatly resembles the bleaching behavior of BB-derived BrC induced by photochemical oxidation and even complex real-world atmospheric processes (Chen et al., 2018; Kumar et al., 2018). For example, OH radical oxidation of woodburning emissions led to the mass absorption cross section at 370 nm of BrC declining by up to 2.3 times (Kumar et al., 2018). The BB BrC AAE values were observed to change during O3 aging as well (Fig. 3d). The RS and CS BrC AAE values generally increased as the O3 oxidation proceeded, suggesting that the light absorption of aged BrC exhibited a stronger wavelength dependence than fresh BrC. This result is consistent with the behavior of BB-derived BrC under photochemically aged processes reported in previous studies (Rincón et al., 2010; Saleh et al., 2013). It is noted that the PW BrC AAE values increased during the first 2 h but gradually declined during the next oxidation period. This behavior might be ascribed to the chromophores in crop straw and woodburning-produced BrC fractions having some differences in their respective compositions, which can be seen in the UV–Vis (Fig. S3) and SF spectra (Fig. S4) as well as those presented in previous studies (Fan et al., 2016, 2018).

3.3 EEM and PARAFAC

As shown in Figs. S5–S7, all the initial EEMs of the BB BrC display two apparent peaks, which are located in the range of λexλem= 230–240 nm/365–390 nm and 260–270 nm/360 nm. These two peaks are usually assigned to tyrosine-like compounds and tryptophan-like compounds, respectively, which are widely found in water-soluble organic matter (WSOM) and humic-like substances (HULISs) in BB aerosols (Fan et al., 2016; Huo et al., 2018), atmospheric aerosols, and rainwater (Qin et al., 2018; Santos et al., 2012). During the O3 aging process, these two fluorescence peaks in the BB BrC EEMs gradually shifted to longer-wavelength regions as the oxidation time increased. After 24 h of O3 aging, the above two peaks had almost disappeared and were replaced by the appearance of two apparent peaks in the range of λexλem= 240–250 nm/400–425 nm and 280–290/390–400 nm for the three BB BrC samples (Figs. S5–S7). These two peaks are usually assigned to humic-like fluorophores (Qin et al., 2018; Santos et al., 2009, 2012), suggesting the formation of new fluorophores in the aged BB BrC. It is obvious that the resulting spectral characteristics are very similar to those of HULISs and WSOM in atmospheric aerosols and rainwater (Qin et al., 2018; Santos et al., 2009, 2012). Therefore, it can be concluded that O3 aging leads to a significant transformation of BB BrC, from dominant protein-like fluorophores within fresh BB BrC to dominant humic-like fluorophores within aged BB BrC.

Quantified variations of the independent fluorophores can be revealed with EEM–PARAFAC. On the basis of a comparison of the PARAFAC-derived components for the atmospheric WSOM and HULISs (Chen et al., 2016a, b), BB-produced WSOM and HULISs (Huo et al., 2018), biochar- and compost-produced dissolved organic matter (DOM) (Jamieson et al., 2014; Huang et al., 2018), and four fluorescent components were identified for the fresh and O3-aged BB BrC extracts. Their profiles and the corresponding assignments are shown in Fig. 4a and Table S1 in the Supplement, respectively. In general, these four individual fluorophores can be attributed to long-wavelength humic-like chromophores (HULIS-1, C1), short-wavelength humic-like chromophores that are less oxygenated (HULIS-2, C2) and highly oxygenated (HULIS-3, C4), and protein-like or phenol-like organic matter (PLOM, C3) (Chen et al., 2016a, b; Gao et al., 2017; Huang et al., 2018; Huo et al., 2018; Jamieson et al., 2014). It is noted that HULIS-1, 2, and 3 are commonly found in the EEMs of HULISs and WSOM in atmospheric aerosols and rainwater (Chen et al., 2016b; Matos et al., 2015), which are expected to consist of fluorophores with strong aromaticity and large molecular sizes. PLOM is mainly comprised of the fluorophores having a similar position of fluorescence peaks to proteins, which generally include nitrogen-containing compounds (e.g., atmospheric amines and amides) and also non-nitrogen-containing species (e.g., phenol- and naphthalene-like substances) (Chen et al., 2016a; Fan et al., 2016; Huo et al., 2018). It is noted that the identified PLOM spectra of the O3-aged BrC resemble to those of fresh BrC, suggesting protein-like and/or phenol-like substances are important fluorophores within both the fresh and aged BB-produced BrC.

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Figure 4(a) Three fluorescent components of the BB BrC identified by the EEM–PARAFAC model. (b) Variations in the abundances of the individual fluorescent components (C1–C4) within the water-soluble BrC in RS, CS, and PW smoke particles during the ozone aging process. The fluorescent spectra of components C1–C4 are listed in the upper layer, and the corresponding loading positions and identification are shown in Table S1.

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The quantitative analysis of the PARAFAC-derived components of the BB BrC upon O3 aging are displayed in Fig. S8. It can be seen that the O3 aging caused significant quenching for components C1–C3 for all the BB BrC samples, in which the quenching of C3 is the most dominant, with a degree range of 71 % to 94 %. These findings suggest that the PLOM is susceptible to O3 aging. Noteworthy, the component C4 exhibits a gradual increase with O3 aging for the RS and CS BrC. This suggests that HULIS-3 is generated accompanied by the decomposition of PLOM, HULIS-1, and HULIS-2 for crop straw burning BrC. In comparison, HULIS-3 (C4) presented an obvious decrease for the PW BrC, indicating that all the fluorophores in the samples were susceptible to O3 aging. The variations in the proportional distribution of the independent components during O3 aging can be observed in Fig. 4b. In terms of the proportion of HULIS-1 (C1), the BrC samples during O3 aging presented relatively higher values than the fresh ones, but there were no significant differences among the aged samples. The proportion of PLOM (C3) within the BB BrC gradually declined during O3 aging, where the total decrease was from 37 % to 16 % for RS, 45 % to 16 % for CS, and 51 % to 13 % for PW. In comparison, the proportion of HULIS-3 (C4) gradually increased from 0 % to 16 % for RS, 11 % to 31 % for CS, and 24 % to 51 % for PW. Taken together, the distributions of the humic-like fluorophores (HULIS-1, 2, 3) accounted for 84 %–87 % of the aged BB BrC, which is much higher than the range 49 %–63 % observed for the initial fresh samples. It had been revealed that the O3 oxidation of oxy-aromatics could produce polyhydroxylated aromatics with light absorption toward longer wavelengths (Lavi et al., 2017; Magalhães et al., 2017; Pillar et al., 2015). These oligomers might have a similar fluorescence peak to those of the humic-like fluorophores, so that the humic-like fluorophores identified herein might also be ascribed to the polyhydroxylated aromatics. These results indicate that humic-like fluorophores or possible polyhydroxylated aromatics in BB BrC are gradually enriched during O3 aging. This is in good agreement with the findings from the enhanced humification of BB BrC with the increased HIX values after O3 aging. Importantly, the resulting humic-like fluorophore distribution is similar to the 84 %–85 % range reported in previous field WSOM measurements (Chen et al., 2016b; Matos et al., 2015), which thus implies that the fluorophore distributions for aged BB BrC are similar to relevant atmospheric distributions. In addition, it is obvious that the quenching behavior of each fluorophore is highly different among the three types of BB BrC during O3 aging. For example, smaller distributions of HULIS-1 and larger distributions of HULIS-3 in the aged PW smoke BrC were found relative to those in the aged crop straw smoke BrC (Fig. 4b). This implies that PW BrC contains less high-molecular-weight HULISs and more low-molecular-weight HULISs with highly oxygenated fluorophores.

3.4 2D-COS combined with fluorescence and FTIR spectra

Two-dimensional correlation spectroscopy was applied to the series of SF and FTIR spectra to tentatively interpret the O3 aging mechanism of BB BrC. As shown in Fig. S9, the major autopeaks centered at 267/289, 284, and 276 nm are present in the RS, CS, and PW BrC synchronous maps, respectively. These peaks are all within the protein-like fluorescent region, suggesting that the protein-like substances in the samples are very susceptible to O3 aging. These results agree well with the significantly reduced PLOM found from the PARAFAC-derived fluorophore analysis. The asynchronous maps further revealed the sequential changes of the fluorophores within the BrC, as illustrated in Fig. S10. According to Noda's rule and the signs of the cross peaks given in Table S2, the order of fluorophore changes is as follows: 267, 289, and 333 nm for the RS BrC; 263, 284, and 340 nm for the CS BrC; and 309, 276, and 358 nm for the PW BrC. The fluorescence regions in the wavelength ranges of 250 to 300 nm and 300 to 380 nm are usually attributed to protein-like and fulvic-like fluorophores, respectively (Chen et al., 2015; Jin et al., 2018; Pantelaki et al., 2018). It is obvious that the RS and CS BrC exhibited the same susceptible fluorophore changes upon O3 aging, showing that the oxidation of the protein-like fraction (263–289 nm) occurred earlier than that of the fulvic-like fraction (333–340 nm). For the PW BrC, the O3 aging of the fulvic-like fluorophores with shorter wavelengths (309 nm) took place before the protein-like fluorophores (276 nm), which was followed by the fulvic-like fluorophores with longer wavelengths (358 nm). The results also imply that the reaction sites in the PW BrC under O3 aging were different from those in crop straw burning BrC. Taken together, it can be concluded that the protein-like substances were susceptible fluorophores, which were primarily oxidized before the longer-wavelength fulvic-like substances. This, however, is in contrast with the results from the dark OH radical oxidation of BB BrC reported in our recent study (Fan et al., 2019), suggesting that the fluorophores within the BrC oxidized by OH in the aqueous phase and those by O3 in the particle phase are different.

https://www.atmos-chem-phys.net/20/4593/2020/acp-20-4593-2020-f05

Figure 5FTIR spectra, and the corresponding synchronous and asynchronous 2D-FTIR-COS maps for the RS (a1–a3), CS (b1–b3), and PW (c1–c3) BrC during the ozone aging process.

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The main changes in the FTIR spectra of the BB BrC upon O3 aging are displayed in the range 1800–1000 cm−1 (Fig. 5). Detailed information regarding any variations in the intensity and sequence of the functional groups upon O3 aging can be revealed with 2D-FTIR-COS (2D-COS combined with FTIR), where synchronous and asynchronous maps were obtained, as shown in Fig. 5. The position and signs of the autopeaks and cross peaks are summarized in Table S3. In general, the synchronous maps exhibit one autopeak at 1725 cm−1 for RS BrC, two autopeaks at 1726 and 1639 cm−1 for CS BrC, and two autopeaks at 1725 and 1630 cm−1 for PW BrC. These findings suggest that carboxylic C=O (1725, 1726 cm−1) and aromatic C=C and C=O groups (1630, 1639 cm−1) (Yan et al., 2013; Fan et al., 2016, 2013; Chen et al., 2015; Zhao et al., 2016) are very susceptible to O3 aging. This result can be confirmed by the evolutionary behavior of O3 oxidation of BB-derived phenolic compounds (Pillar et al., 2014, 2015, 2017). For example, O3 primarily attacked the C=C bond in such oxy-aromatics to yield polyfunctional low-molecular-weight carboxylic acids containing C=O groups (Pillar et al., 2014, 2015, 2017). In addition, the cross peaks generally showed a positive correlation between the series of bands at 1725 (1726), 1639, 1630, 1400, 1318, and 1224 (1211) cm−1. This implies that the stretching of carboxylic C=O (1725, 1726 cm−1); phenol-OH (1400, 1224, 1211 cm−1); and aromatic C=C, ketone, and amide C=O groups (1630, 1639 cm−1) (Yan et al., 2013; Fan et al., 2016, 2013; Chen et al., 2015; Zhao et al., 2016; Wang et al., 2017) was cotransformed upon O3 aging. It is also in good agreement with the ozonolysis pathway for BB-derived oxy-aromatics (Pillar et al., 2014, 2015, 2017). For example, the O3 oxidation of oxy-aromatics can cause the cleavage of the aromatic bond (C=C) to generate polyfunctional low-molecular-weight carboxylic acids (C=O) and also formation of polyhydroxylated aromatics (phenol-OH). Moreover, the cross peaks also presented a positive correlation between another series of bands at 1585 (1580) and 1515 cm−1, suggesting that the vibration of aromatic COO (1580, 1585 cm−1) and lignin skeletal C=C (1515 cm−1) (Fan et al., 2016; Yan et al., 2013; Wang et al., 2017) was also changed in the same way. However, there is a negative correlation between these two series of bands, implying that the spectral changes proceeded in reverse. Given the changes seen in the FTIR spectra (Fig. 5a1–c1), we can conclude that the former groups (i.e., carboxylic C=O, phenol-OH, protein C=C and C=O, and aromatic ketone C=O groups) are more likely to be generated, while the lignin structures (i.e., aromatic COO and lignin skeletal C=C) tend to be decomposed. Actually, this is consistent with observations of more oxygen-containing functional groups (i.e., ketones, aldehydes, and anhydrides) that formed in soot particles after O3 oxidation reported in many previous studies (He et al., 2017; Li et al., 2013, 2015). For example, He et al. (2017) pointed out that O3-aged soot particles presented an increase in the intensities of the bands at 1715, 1630, and 1055 cm−1 in their FTIR spectra, suggesting the formation of ketone C=O and C–O groups. The increase in oxygen-containing functional groups might lead to the solubility of OC, which is in good agreement with the former observations of much more WSOC being generated during the O3 aging of BB smoke particles.

The asynchronous maps displayed more information regarding the changes in the functional groups. The major cross peaks and their signs are listed in Table S3. According to Noda's rule, the sequence of bands upon O3 aging is as follows: 1224, 1725, 1580, and 1515 cm−1 for the RS BrC; 1400, 1211, 1726, 1639, and 1515 cm−1 for the CS BrC; and 1630, 1725, 1585, 1515, and 1318 cm−1 for the PW BrC. These indicate that different functional groups within the BrC formed or changed during O3 aging. Nevertheless, the similar structural change sequence of the BB BrC functional groups upon O3 aging could be ascertained. The changes follow the order of aromatic OH (1400, 1224, 1211 cm−1); conjugated C=O groups (1725, 1726 cm−1) and aromatic C=O (1630, 1639 cm−1); aromatic COO (1585 and 1580 cm−1); and lignin-derived C–C, C–H, and C–O groups (1515 and 1318 cm−1). Actually, the conjugated C=O formation was observed to have occurred before aromatic C=O formation for the CS BrC (1726>1639 cm−1) during O3 aging, while the reverse sequences were observed for the PW BrC (1630>1725 cm−1). These results suggest that the aromatic C=O functional groups involved with O3 aging are different for these two types of BB BrC. As revealed by our previous study, the pyrolysis of CS and PW HULISs dominantly generated lignin derivatives, but amounts of diterpenoid derivatives were also formed by pyrolysis of PW HULISs (Fan et al., 2016). Therefore, it can be speculated that the 1639 cm−1 feature can more likely be attributed to the stretching of aromatic C=O, linked to the lignin derivative of the CS BrC, while the 1630 cm−1 feature can more likely be ascribed to the stretching of aromatic C=C and C=O, linked to the diterpenoid derivatives of the PW BrC.

4 Conclusions and atmospheric implications
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In this study, the O3 aging of BB smoke particles from the burning of RS, CS, and PW was conducted to investigate the evolutionary behavior of BrC. The results showed that the WSOC content of BB BrC quickly increased during the initial O3 aging period but slowly decreased during the final O3 aging period. Simultaneously, the O3 aging led to a gradual reduction of absorption and fluorescence for all the BB BrC samples, suggesting the significant degradation of chromophores and fluorophores. Moreover, a decrease in aromaticity and light-absorbing ability of the chromophores and an increase in humification of the fluorophores within the BB BrC were also observed during the aging process. Therefore, it might be concluded that both the degradation and formation of water-soluble OC fractions occur during O3 aging.

EEM–PARAFAC revealed that HULIS-1, HULIS-2, and PLOM within the BB samples were significantly degraded, but newly formed HULIS-3 was obviously observed for two of the RS and CS samples. In addition, the relative distribution of PLOM gradually decreased, and that of HULIS-3 was observed to gradually increase for all the BB BrC samples. This indicates that the aged BB BrC contains more humic-like substances with highly oxygenated chromophores but fewer protein-like substances. These results also confirm that the fluorophore composition of BrC is altered by the O3 aging of the BB particles, and more humic-like substances (HULIS-1, 2, 3) are abundant in aged BB BrC. It is noted that the fluorescent components of the aged BB BrC are quite similar to those of atmospheric BrC.

Two-dimensional correlation spectroscopy of the SF and FTIR spectra revealed valuable information regarding the reactive positions and sequences within BB BrC for O3 aging. Although the different chemical compositions of BB BrC resulted in different evolutionary behavior, some similar O3 aging mechanisms could be tentatively identified. The results from 2D-SF-COS analysis suggest that the protein-like fraction (263–289 nm) is susceptible to O3 aging before the fulvic-like fraction (333–340 nm) within the RS and CS BrC. The PW BrC presented susceptible fluorophores in the order of fulvic-like fluorophores with shorter wavelengths (309 nm), protein-like fluorophores (276 nm), and fulvic-like fluorophores with longer wavelengths (358 nm). The 2D-FTIR-COS analysis revealed the sequence of aromatic OH (1400, 1224, 1211 cm−1); conjugated C=O groups (1725 cm−1) and aromatic C=O (1630, 1639 cm−1); aromatic COO (1585 and 1580 cm−1); and lignin-derived C–C, C–H, and C–O groups (1515 and 1318 cm−1) for all the BB BrC. This implies that the formation of phenols occurred before the formation of carboxylic C=O and aromatic C=O through O addition, which were then accompanied by the decomposition of lignin derivatives for all the BB BrC during O3 aging.

Given the high concentrations of O3 and large amounts of BB emission in the atmosphere, O3 aging processes often occur in the atmospheric environment. The results obtained in this study provide new insights into the evolutionary behavior of the chromophoric and fluorescent properties of BB BrC during O3 aging, which have important implications in terms of the heterogeneous oxidation of BB BrC. Furthermore, previous modeling studies related to BB BrC only focused on parameterizing the optical properties of fresh BB BrC, and the consideration of effects related to atmospheric processes on BB BrC is limited. The present study revealed that a considerable bleaching rather than competitive formation of chromophoric BrC occurred during O3 aging of BB smoke samples, indicating that the relevant oxidation chemistry taking place in the atmospheric gas phase will weaken the light absorption properties of BB-derived BrC. As a result, the radiative forcing potential of BB-derived BrC is likely overestimated. These findings are of great importance for improving the accuracy of climate models as well as source apportionment models that consider the optical properties of BrC. However, some questions still remain and more studies should be conducted in the future on (1) the O3 oxidation mechanism of BB BrC under different conditions (e.g., O3 concentration, RH, temperature), especially under real atmospheric environment, and (2) the O3 oxidation mechanism of BrC derived from other sources such as fossil fuel combustion and secondary chemical formation.

Data availability
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Data availability. 

All data needed to evaluate the conclusions in the paper are present in the paper and Supplement. Additional data related to this paper may be requested from the authors.

Supplement
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Supplement. 

The supplement related to this article is available online at: https://doi.org/10.5194/acp-20-4593-2020-supplement.

Author contributions
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Author contributions. 

JS, PP, and XF conceived of the experiment. TC and XY built the ozone aging reactor and operated the ozone aging of biomass burning fine particles. TC, XY, YW, and WJ performed the TOC, UV–Vis, 3DEEM, and FTIR measurements. XF and JS performed 2D-COS and PARAFAC. XF interpreted all data with the assistance of JS. XX, FL, YW, and YX provided useful comments on the paper. XF wrote the paper with the assistance of JS and PP.

Competing interests
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Competing interests. 

The authors declare that they have no conflict of interest.

Special issue statement
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Special issue statement. 

This article is part of the special issue “Multiphase chemistry of secondary aerosol formation under severe haze”. It is not associated with a conference.

Acknowledgements
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Acknowledgements. 

The authors acknowledge the editor and anonymous reviewers for their constructive suggestions.

Financial support
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Financial support. 

This research has been supported by the Natural Science Foundation of China (grant nos. 41705107 and 41673117), the Anhui Science and Technology Major Project (grant no. 16030701102), and the Anhui Provincial Natural Science Foundation (grant no. 1808085MB49).

Review statement
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Review statement. 

This paper was edited by Jian Wang and reviewed by two anonymous referees.

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A particle-phase O3 oxidation of biomass burning (BB) smoke particles was conducted to investigate the evolution of brown carbon (BrC) therein. The O3 aging induced the decomposition of chromophores and fluorophores. EEM–PARAFAC showed a predominant decomposition of protein-like substances and a gradual increase proportion of humic-like substances for BB BrC during O3 aging. The dynamic spectral behavior of chromophores and functional groups within BrC was further explored by 2D-COS.
A particle-phase O3 oxidation of biomass burning (BB) smoke particles was conducted to...
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