Our understanding of the impact of aerosol particles on deep-convective clouds is still incomplete. Earlier research found increased lightning above shipping lanes and hypothesized that the ship emissions support the development of deep-convective cloud systems. The present study investigates the effect of a sevenfold reduction in sulphur content of shipping fuel, implemented following the International Marine Organisation 2020 regulation. A significant decrease in lightning activity is found over shipping lanes in combination with a reduction of both the concentration and the size of the emitted particles, providing further evidence of a strong connection between shipping missions, deep-convective cloud development, and lightning activity. This highlights the need to resolve the still unclear acting mechanisms.