The initiation of autoxidation by ozonolysis was investigated on levitated linoleic acid droplets using electrodynamic balance–mass spectrometry. Exposing the droplets to ozone for 1 h before switching the gas phase to air without ozone led to a shortening of the autoxidation initiation phase in comparison to experiments without ozone exposure. Results were compared to a bulk reaction model to investigate the synergistic effects of ozonolysis and autoxidation.