Articles | Volume 20, issue 3
https://doi.org/10.5194/acp-20-1817-2020
© Author(s) 2020. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
https://doi.org/10.5194/acp-20-1817-2020
© Author(s) 2020. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
Research article
| 
14 Feb 2020
Research article |
| 14 Feb 2020
| 14 Feb 2020
Using flow cytometry and light-induced fluorescence to characterize the variability and characteristics of bioaerosols in springtime in Metro Atlanta, Georgia
Arnaldo Negron
School of Earth and Atmospheric Sciences, Georgia Institute of
Technology, Atlanta, GA 30332, USA
School of Chemical and Biomolecular Engineering, Georgia Institute of
Technology, Atlanta, GA 30332, USA
Natasha DeLeon-Rodriguez
School of Biology, Georgia Institute of Technology, Atlanta, GA
30332, USA
currently at: Puerto Rico Science, Technology and
Research Trust, Rio Piedras, 00927, Puerto Rico
Samantha M. Waters
School of Earth and Atmospheric Sciences, Georgia Institute of
Technology, Atlanta, GA 30332, USA
currently at: Department of Marine Sciences,
University of Georgia, Athens, GA 30602-3636, USA
Luke D. Ziemba
School of Biological Sciences, Chemistry and Dynamics Branch/Science Directorate, National
Aeronautics and Space Administration Langley Research Center,
Hampton, VA 23681, USA
Bruce Anderson
School of Biological Sciences, Chemistry and Dynamics Branch/Science Directorate, National
Aeronautics and Space Administration Langley Research Center,
Hampton, VA 23681, USA
Michael Bergin
Department of Civil and Environmental Engineering, Duke
University, Durham, NC 2770, USA
Konstantinos T. Konstantinidis
CORRESPONDING AUTHOR
School of Civil and Environmental Engineering, Georgia
Institute of Technology, Atlanta, GA 30332, USA
School of Biology, Georgia Institute of Technology, Atlanta, GA
30332, USA
Athanasios Nenes
CORRESPONDING AUTHOR
School of Earth and Atmospheric Sciences, Georgia Institute of
Technology, Atlanta, GA 30332, USA
Institute for Chemical Engineering Science, Foundation
for Research and Technology Hellas, Patra, 26504, Greece
Laboratory of Atmospheric Processes and their Impacts
(LAPI), School of Architecture, Civil & Environmental
Engineering, Ecole Polytechnique Fédérale de
Lausanne, Lausanne, 1015, Switzerland
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Cited
15 citations as recorded by crossref.
- Detection and characterization of bioaerosol emissions from wastewater treatment plants: Challenges and opportunities J. Tian et al. https://doi.org/10.3389/fmicb.2022.958514
- Rapid quantification of bacterial aerosol abundance and influencing factors in high altitude regions based on optimized flow cytometry G. Mao et al. https://doi.org/10.1016/j.atmosres.2025.108696
- Biological and dust aerosols as sources of ice-nucleating particles in the eastern Mediterranean: source apportionment, atmospheric processing and parameterization K. Gao et al. https://doi.org/10.5194/acp-24-9939-2024
- Assessing the global contribution of marine aerosols, terrestrial bioaerosols, and desert dust to ice-nucleating particle concentrations M. Chatziparaschos et al. https://doi.org/10.5194/acp-25-9085-2025
- Atmospheric Biodetection Part I: Study of Airborne Bacterial Concentrations from January 2018 to May 2020 at Saclay, France R. Sarda-Estève et al. https://doi.org/10.3390/ijerph17176292
- Biogenic and Anthropogenic Secondary Organic Aerosols Become Fluorescent after Highly Acidic Aging C. Wong et al. https://doi.org/10.1021/acs.jpca.4c04287
- Biological and Nonbiological Sources of Fluorescent Aerosol Particles in the Urban Atmosphere S. Yue et al. https://doi.org/10.1021/acs.est.1c07966
- A systematic review of two LIF-based instruments for bioaerosol monitoring: Data treatment, performance evaluation, applications and comparison of WIBS and Rapid-E A. Carminati et al. https://doi.org/10.1016/j.atmosenv.2026.122220
- Flow cytometric analysis of pollen and spores: An overview of applications and methodology P. Kron et al. https://doi.org/10.1002/cyto.a.24330
- Characterization of the atmospheric microbiome in a semi-rural area of Central Europe using flow cytometry E. Abboud et al. https://doi.org/10.1093/ismeco/ycag167
- Bioaerosol Emission Characteristics from Laboratory Burns A. Shawon et al. https://doi.org/10.1021/acsestair.4c00310
- Challenges and Perspectives for Biosensing of Bioaerosol Containing Pathogenic Microorganisms M. Li et al. https://doi.org/10.3390/mi12070798
- A Modified Spectroscopic Approach for the Real-Time Detection of Pollen and Fungal Spores at a Semi-Urban Site Using the WIBS-4+, Part I E. Markey et al. https://doi.org/10.3390/s22228747
- Interdisciplinary strategy to assess the impact of meteorological variables on the biochemical composition of the rain and the dynamics of a small eutrophic lake under rain forcing F. Noirmain et al. https://doi.org/10.5194/bg-19-5729-2022
- Mesoporous TiO2 Monoliths Impregnated with CdS and CuO Nanoparticles for Airborne Bacteria Inactivation Under Visible Light A. Hernández-Gordillo & S. Arriaga https://doi.org/10.1007/s10562-021-03659-9
15 citations as recorded by crossref.
- Detection and characterization of bioaerosol emissions from wastewater treatment plants: Challenges and opportunities J. Tian et al. https://doi.org/10.3389/fmicb.2022.958514
- Rapid quantification of bacterial aerosol abundance and influencing factors in high altitude regions based on optimized flow cytometry G. Mao et al. https://doi.org/10.1016/j.atmosres.2025.108696
- Biological and dust aerosols as sources of ice-nucleating particles in the eastern Mediterranean: source apportionment, atmospheric processing and parameterization K. Gao et al. https://doi.org/10.5194/acp-24-9939-2024
- Assessing the global contribution of marine aerosols, terrestrial bioaerosols, and desert dust to ice-nucleating particle concentrations M. Chatziparaschos et al. https://doi.org/10.5194/acp-25-9085-2025
- Atmospheric Biodetection Part I: Study of Airborne Bacterial Concentrations from January 2018 to May 2020 at Saclay, France R. Sarda-Estève et al. https://doi.org/10.3390/ijerph17176292
- Biogenic and Anthropogenic Secondary Organic Aerosols Become Fluorescent after Highly Acidic Aging C. Wong et al. https://doi.org/10.1021/acs.jpca.4c04287
- Biological and Nonbiological Sources of Fluorescent Aerosol Particles in the Urban Atmosphere S. Yue et al. https://doi.org/10.1021/acs.est.1c07966
- A systematic review of two LIF-based instruments for bioaerosol monitoring: Data treatment, performance evaluation, applications and comparison of WIBS and Rapid-E A. Carminati et al. https://doi.org/10.1016/j.atmosenv.2026.122220
- Flow cytometric analysis of pollen and spores: An overview of applications and methodology P. Kron et al. https://doi.org/10.1002/cyto.a.24330
- Characterization of the atmospheric microbiome in a semi-rural area of Central Europe using flow cytometry E. Abboud et al. https://doi.org/10.1093/ismeco/ycag167
- Bioaerosol Emission Characteristics from Laboratory Burns A. Shawon et al. https://doi.org/10.1021/acsestair.4c00310
- Challenges and Perspectives for Biosensing of Bioaerosol Containing Pathogenic Microorganisms M. Li et al. https://doi.org/10.3390/mi12070798
- A Modified Spectroscopic Approach for the Real-Time Detection of Pollen and Fungal Spores at a Semi-Urban Site Using the WIBS-4+, Part I E. Markey et al. https://doi.org/10.3390/s22228747
- Interdisciplinary strategy to assess the impact of meteorological variables on the biochemical composition of the rain and the dynamics of a small eutrophic lake under rain forcing F. Noirmain et al. https://doi.org/10.5194/bg-19-5729-2022
- Mesoporous TiO2 Monoliths Impregnated with CdS and CuO Nanoparticles for Airborne Bacteria Inactivation Under Visible Light A. Hernández-Gordillo & S. Arriaga https://doi.org/10.1007/s10562-021-03659-9
Saved (final revised paper)
Latest update: 21 Sep 2026
Short summary
Airborne biological particles impact human health, cloud formation, and ecosystems, but few techniques are available to characterize their atmospheric abundance. Combining a newly developed high-volume sampling/flow cytometry technique together with an laser-induced fluorescence instrument, we detect a highly dynamic bioaerosol community over urban Atlanta, composed of pollen, fungi, and bacteria with low and high nucleic acid content.
Airborne biological particles impact human health, cloud formation, and ecosystems, but few...
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