The absorption cross section of N₂O₅, &sigma;_N₂O₅(&lambda;, <i>T</i>), which is known from laboratory measurements with the uncertainty of a factor of 2 (Table 4-2 in (Jet Propulsion Laboratory) JPL-2011; the spread in laboratory data, however, points to an uncertainty in the range of 25 to 30%, Sander et al., 2011), was investigated by balloon-borne observations of the relevant trace gases in the tropical mid-stratosphere. The method relies on the observation of the diurnal variation of NO₂ by limb scanning DOAS (differential optical absorption spectroscopy) measurements (Weidner et al., 2005; Kritten et al., 2010), supported by detailed photochemical modelling of NO_y (NO_x(= NO + NO₂) + NO₃ + 2N₂O₅ + ClONO₂ + HO₂NO₂ + BrONO₂ + HNO₃) photochemistry and a non-linear least square fitting of the model result to the NO₂ observations. Simulations are initialised with O₃ measured by direct sun observations, the NO_y partitioning from MIPAS-B (Michelson Interferometer for Passive Atmospheric Sounding – Balloon-borne version) observations in similar air masses at night-time, and all other relevant species from simulations of the SLIMCAT (Single Layer Isentropic Model of Chemistry And Transport) chemical transport model (CTM). Best agreement between the simulated and observed diurnal increase of NO₂ is found if the &sigma;_N₂O₅(&lambda;, <i>T</i>) is scaled by a factor of 1.6 ± 0.8 in the UV-C (200–260 nm) and by a factor of 0.9 ± 0.26 in the UV-B/A (260–350 nm), compared to current recommendations. As a consequence, at 30 km altitude, the N₂O₅ lifetime against photolysis becomes a factor of 0.77 shorter at solar zenith angle (SZA) of 30&deg; than using the recommended &sigma;_N₂O₅(&lambda;, <i>T</i>), and stays more or less constant at SZAs of 60&deg;. Our scaled N₂O₅ photolysis frequency slightly reduces the lifetime (0.2–0.6%) of ozone in the tropical mid- and upper stratosphere, but not to an extent to be important for global ozone.