Dimer esters in α-pinene secondary organic aerosol : e ff ect of hydroxyl radical , ozone , relative humidity and aerosol acidity

Introduction Conclusions References


Abstract
The formation of secondary organic aerosol (SOA) from both ozonolysis and hydroxyl radical (OH)-initiated oxidation of α-pinene under conditions of high nitric oxide (NO) concentrations with varying relative humidity (RH) and aerosol acidity was investigated in the University of North Carolina dual outdoor smog chamber facility.SOA formation from ozonolysis of α-pinene was enhanced relative to that from OH-initiated oxidation in the presence of initially high NO conditions.However, no effect of RH on SOA mass was evident.Ozone (O 3 )-initiated oxidation of α-pinene in the presence of ammonium sulfate (AS) seed coated with organic aerosol from OH-initiated oxidation of α-pinene showed reduced nucleation compared to ozonolysis in the presence of pure AS seed aerosol.
The chemical composition of α-pinene SOA was investigated by ultra-performance liquid chromatography/electrospray ionization high-resolution quadrupole time-of-flight mass spectrometry (UPLC/ESI-HR-Q-TOFMS), with a focus on the formation of carboxylic acids and high-molecular weight dimer esters.A total of eight carboxylic acids and four dimer esters were identified, constituting between 8 and 12 % of the total α-pinene SOA mass.OH-initiated oxidation of α-pinene in the presence of nitrogen oxides (NO x ) resulted in the formation of highly oxidized carboxylic acids, such as 3methyl-1,2,3-butanetricarboxylic acid (MBTCA) and diaterpenylic acid acetate (DTAA).The formation of dimer esters was observed only in SOA produced from the ozonolysis of α-pinene in the absence of NO x , with increased concentrations by a factor of two at higher RH (50-90 %) relative to lower RH (30-50 %).The increased formation of dimer esters correlates with an observed increase in new particle formation at higher RH due to nucleation.Increased aerosol acidity was found to have a negligible effect on the formation of the dimer esters.SOA mass yield did not influence the chemical composition of SOA formed from α-pinene ozonolysis with respect to carboxylic acids and dimer esters.

Introduction
Formation of biogenic secondary organic aerosol (BSOA) through atmospheric processing of naturally emitted biogenic volatile organic compounds (BVOCs) comprises a major fraction of secondary organic aerosol (SOA) in the atmosphere (Hallquist et al., 2009).Monoterpenes (C 10 H 16 ), including α-pinene, constitute an important group of BVOCs emitted from coniferous trees as well as other types of vegetation (Guenther et al., 1995).Once released, α-pinene is quickly oxidized by hydroxyl radical (OH) in the sunlit atmosphere, reaction with ozone (O 3 ), and during night-time, with nitrate radical (NO 3 ), resulting in a complex mixture of first-generation oxidation products consisting primarily of carbonyls, carboxylic acids, alcohols, and organic nitrates (Warneke et al., 2004;Yu et al., 1999;Glasius et al., 2000;Larsen et al., 2001;Hallquist et al., 2009;Camredon et al., 2010;Perraud et al., 2010;Fry et al., 2009).Due to the lower vapour pressure of the oxidation products, they partition between the gas and particle phases, resulting in the formation of SOA (Kroll and Seinfeld, 2008;Odum et al., 1996;Hoffmann et al., 1997;Donahue et al., 2006;Jimenez et al., 2009) O 3 oxidation of α-pinene proceeds via addition across the double bond, leading to formation of an energy-rich primary ozonide (Fig. 1).The ozonide decomposes rapidly through one of two channels, each forming an energy-rich Criegee intermediate.The Criegee intermediates are either collisionally stabilised (sCI channel, Fig. 1) or decompose to yield OH and an additional organic radical (hydroperoxide channel, Fig. 1).
Although several reactions are possible for sCI, reaction with water is believed to predominate under atmospheric conditions (Atkinson and Arey, 1998;Kroll and Seinfeld, 2008), leading to formation of an α-hydroxy hydroperoxide and other first-generation oxidation products such as pinonaldehyde and pinonic acid (Jenkin et al., 2000;Kroll and Seinfeld, 2008).In addition, the sCI may also form a secondary ozonide through the reaction with carbonyl compounds (Fig. 1).Other important and more highly oxidized products, such as 10-hydroxy-pinonic and pinic acids, are formed from the decomposition of more highly substituted Criegee biradicals (Jenkin et al., 2000).
The first-generation oxidation products of α-pinene may undergo further processing in the gas and aerosol phases, such as oxidation (Szmigielski et al., 2007;Müller et al., 2012), sulfation (Surratt et al., 2008;Iinuma et al., 2007), and oligomerization (Tolocka et al., 2004;Gao et al., 2004;Kalberer et al., 2004), resulting in the formation of less volatile, high-molecular weight compounds.For example, pinonic acid is semivolatile and a significant fraction of pinonic acid is present in the gas phase at 283 K (Müller et al., 2012).Through further reaction with OH, gas-phase pinonic acid is oxidized to the second-generation oxidation product 3-methyl-1,2,3-butane tricarboxylic acid (MBTCA) (Szmigielski et al., 2007;Zhang et al., 2010;Müller et al., 2012), which has low vapour pressure and rapidly partitions into the aerosol phase.Once condensed in the aerosol phase, other first-generation compounds, such as pinic and terpenylic acids, are believed to undergo oligomerization to form dimers (Yasmeen et al., 2010), which are effectively captured because of their low vapour pressure.
Species having compositions corresponding to structures tentatively identified as dimer esters originating from α-and β-pinene have been observed in both laboratorygenerated and ambient SOA (Müller et al., 2008Müller et al., 2009;Camredon et al., Introduction Conclusions References Tables Figures

Back Close
Full 2010; Yasmeen et al., 2010;Gao et al., 2010;Kristensen et al., 2013).Yasmeen et al. (2010) presented the first field measurements of pinyl-diaterpenyl ester (MW 358), diaterpenyl-terpenyl ester (MW 344), and pinyl-diaterebyl ester (MW 344) in ambient aerosols collected during warm nights at K-Puszta, Hungary.In addition, the pinyl-diaterpenyl ester along with pinonyl-pinyl ester (MW 368) have also recently been identified in both day-time and night-time aerosols collected from the Blodgett Forest in the Sierra Nevada Mountains, USA, at concentrations comparable to some first-and second-generation oxidation products from α-pinene (Kristensen et al., 2013).The formation of the pinyl-diaterpenyl ester was explained by Yasmeen et al. (2010) as an esterification of cis-pinic acid with diaterpenylic acid, which was proposed to be generated as an intermediate by acid-catalyzed hydrolysis of the α-pinene oxidation products, terpenylic acid and/or diaterpenylic acid acetate (Yasmeen et al., 2010).The two MW 344 dimer esters have been hypothesized to originate from the esterification of terpenylic acid and diaterpenylic acid (diaterpenyl-terpenyl ester), and from diaterebic acid, a homolog of diaterpenylic acid, and cis-pinic acid (pinyl-diaterebyl ester).
The pinonyl-pinyl ester is hypothesized to form from the esterification of cis-pinic and 10-hydroxy-pinonic acids (Yasmeen et al., 2010).Hence, these dimer esters have been tentatively proposed to be later-generation products formed through esterification in the aerosol phase.In contradiction to this hypothesis, Heaton et al. (2007) observed almost immediate formation of dimers and higher order oligomers in a flow tube study involving the ozonolysis of monoterpenes.Furthermore, a recent study has shown that dimer esters from α-pinene ozonolysis are formed within the same timeframe as their structural precursors (Kristensen et al., 2013).Using on-line atmospheric pressure chemical ionization mass spectrometry (APCI-MS) with high time-resolution, Müller et al. (2008) observed that the intensity of dimer esters increased more rapidly than the intensity of their monomer precursors in freshly nucleated aerosol in the α-pinene/O 3 system.This result led to the suggestion that gas-phase ester formation could be important for homogeneous nucleation, although the exact reaction mechanism has remained elusive.Altogether, the formation mechanisms of dimer esters observed in fine aerosol Introduction

Conclusions References
Tables Figures

Back Close
Full recently collected from both K-Puszta, Hungary (Yasmeen et al., 2010), and in the Blodgett Forest, USA (Kristensen et al., 2013), have yet to be elucidated.
In the present study, we address the formation of several first-and second-generation oxidation products along with four individual dimer esters formed from the oxidation of α-pinene.We investigate the effect of oxidant species (OH vs. O 3 ), relative humidity (RH), and seed aerosol acidity in order to obtain a better understanding of the conditions leading to the formation of the dimer esters and how these parameters may affect the formation and chemical composition of SOA.We propose that the α-pinene-derived dimer esters may arise via gas-phase reactions involving stabilized Criegee intermediates (CI) from the ozonolysis of α-pinene rather than esterification in the aerosol phase, which is kinetically inconsistent with the experimentally observed time scale for dimer formation.

Smog chamber experiments
A total of 12 sets of smog chamber experiments were performed at the University of North Carolina 274 m 3 dual outdoor smog chamber facility (Pittsboro, NC), consisting of two individual chambers with identical volumes (136 m 3 ) divided by a Teflon film curtain.Details of the chamber instrumentation have been described elsewhere (Lee et al., 2004;Leungsakul et al., 2005;Kamens et al., 2011;Zhang et al., 2011;Zhang et al., 2012).During all experiments, O 3 and oxides of nitrogen (NO x ) were measured continuously by UV photometric (ThermoEnvironmental 49P) and chemiluminescent monitors (Bendix Model 8101B analyzers), respectively.α-pinene concentrations were measured by a gas chromatography/flame ionization detector (GC/FID, Model CP-3800, Varian).Particle size distributions and volume concentrations were measured using a scanning mobility particle sizer (SMPS) (TSI 3080) coupled with a condensation particle counter (CPC) (TSI 3022A).All SMPS data were corrected for wall-loss Introduction

Conclusions References
Tables Figures

Back Close
Full rate estimated for each individual experiment by monitoring the particle mass for approximately 2 h after addition of seed particles.For each experiment, aerosol filter samples (PALL Life Sciences, Teflon, 47 mm diameter, 1.0 µm pore size) for off-line ultraperformance liquid chromatography/electrospray ionization high-resolution quadrupole time-of-flight mass spectrometry (UPLC/ESI-HR-Q-TOFMS) analysis were collected at a flow rate of ∼ 17 L min −1 from both chambers.In all experiments, background aerosol filter samples were collected from both chambers prior to the injection of α-pinene.α-pinene was added to both chambers simultaneously by vaporizing a known volume of α-pinene (99 %, Aldrich) in a U-tube manifold into a N 2 flow.Filter sampling of SOA from both chambers was initiated simultaneously after the aerosol volume concentration had reached its maximum value (as determined by the SMPS) and α-pinene was no longer detectable by GC/FID.Table 1 shows the conditions for all experiments.The experiments were conducted on clear sunny days from late July through early December 2012, as reflected in the variation of temperatures for the experiments.The general procedure for experiments was as follows.Both sides of the chamber were vented with rural background air before each experiment.Drying was subsequently performed with a 250 L min −1 Aadco clean air generator at a flow rate of 6 m 3 h −1 for a minimum of 12 h for each side of the chamber.
To investigate the SOA formation from α-pinene at very low-NO x conditions, indoor chamber experiments were conducted in an indoor 10 m 3 flexible Teflon chamber at the UNC Department of Environmental Sciences and Engineering.The chamber was flushed with high-purity air from a clean air generator before the addition seed aerosol by nebulization.Glass microliter syringes were used to inject a known amount of αpinene into a 10 mL glass manifold wrapped with calibrated heating tapes.The tapes were heated to 60 • C while flushing the manifold with high-purity N 2 (preheated to 60 • C) at 5 L min −1 for at least 2 h until no additional increase in aerosol volume was observed by SMPS after which particle samples were collected on 47 mm diameter, 1.0 µm pore size Teflon membrane filters (Pall Life Science) for product analyses, at a sampling flow rate of ∼ 20 L min −1 for 1.5 h.α-pinene ozonolysis experiments with lower SOA mass yield were performed to examine the effect of SOA mass on the formation of organic acid and dimer ester.Here, the two chambers were prepared with similar RH (∼ 90 %), AS seed aerosol (∼ 30 µg m −3 ), O 3 (∼ 70 ppb) and cyclohexane (∼ 16 ppm) concentrations before the addition of 20 ppb of α-pinene to both chambers after sunset.
In addition, the effect of aerosol acidity on the formation of organic acids and dimer esters was also investigated.The two chambers were prepared with similar RH and injected with approximately 30 µg m −3 of seed aerosol.For acidic conditions, a solution of 0.06 M MgSO 4 with 0.06 M H 2 SO 4 was nebulized into the chamber.For neutral conditions, a 0.06 M AS solution was nebulized into the chamber.Aerosol mass concentrations were monitored for ∼ 2 h before ∼ 90 ppb of O 3 was added to both chambers followed by addition of 50 ppb of α-pinene.Introduction

Conclusions References
Tables Figures

Back Close
Full α-pinene SOA formation under conditions with very low (below detection limit) NO x was investigated in the UNC indoor smog chamber.After the addition of ∼ 70 µg m −3 AS seed aerosol, 170 ppb ozone was added to the chamber along with 100 ppb of α-pinene.The experiment was carried out in the dark at a constant temperature (24-25 • C) under dry (RH < 6 %) conditions.No OH-scavenger was added to the chamber.

Effect of RH on SOA from OH oxidation
The formation of SOA from OH-initiated oxidation of α-pinene at different RH was investigated in the outdoor dual chamber.Three days of drying was performed in one chamber to obtain low-RH conditions, while a high-RH atmosphere was obtained by using the clean air generator for only 1-2 h prior to the experiment (temperature and RH profiles are shown in Supplement, Fig. S2).SMPS measurements showed background aerosol mass concentrations less than 5 µg m −3 in the high-RH chamber and less than 2 µg m −3 in the low-RH chamber.Approximately 30 µg m −3 of seed aerosol was injected into each chamber by using a nebulizer that contained a 0.06 M AS solution.
Approximately 200 ppb nitric oxide (NO) was then injected into both chambers from a high-pressure gas cylinder (1 % nitric oxide in nitrogen, Airgas National Welders) and allowed to stabilize.A relatively high level of NO was chosen to effectively titrate any remaining O 3 left in the chambers.100 ppb α-pinene was then added to both chambers simultaneously.For comparison, one experiment (Exp. 1, Table 1) involved the addition of 200 ppb of α-pinene to both chamber.
To investigate how SOA from OH-initiated oxidation of α-pinene would affect further SOA formation from ozonolysis, an injection of 50 ppb α-pinene was made into each chamber after sunset in four of the experiments described above.At the time of the second α-pinene injection, there was no residual α-pinene and the chambers contained 80-200 ppb of O 3 formed from the OH-initiated photooxidation of α-pinene during the day along with the initial AS seed modified by condensation of OH-initiated α-pinene oxidation products.The second injection of α-pinene was made after sunset to minimize further oxidation by OH.The mixtures were shaken and sonicated for 5 min and stored at −20 • C before analysis.
Samples were analyzed using an Agilent Ultra Performance Liquid Chromatography (UPLC) coupled to the electrospray ionization (ESI) inlet of an Agilent 6520 Series Accurate Mass Quadrupole Time-of-Flight Mass Spectrometer (Q-TOFMS) operated in the negative (-) ion mode.The operating conditions have been described in detail elsewhere (Zhang et al., 2011).Chromatograms of identified compounds are shown in Supplement, Fig. S1.
Organic acids and dimer esters identified in the extracted aerosol samples were quantified using cis-pinic acid (a diacid; Sigma-Aldrich), cis-pinonic acid and ketopinic acid (monoacids; Sigma-Aldrich) as surrogates.Six-point calibration curves of the acid surrogates were constructed over the range 0.1-100 µg mL −1 .Because authentic standards were not available, acids having two or more carboxylate groups, including pinic acid, diaterpenylic acid, diaterpenylic acid acetate (DTAA), 3-methyl-butane tricarboxylic acid (MBTCA) and the dimer esters, were quantified using cis-pinic acid, while the monoacids pinonic and hydroxy-pinonic acid where quantified using cis-pinonic acid (Table 2).Based on structural similarity, terebic acid and terpenylic acid were quantified with keto-pinic acid.UPLC/ESI-HR-Q-TOFMS analysis of all background aerosol filters collected from each set of experiments indicated that both chambers were free of α-pinene SOA constituents prior to injection of α-pinene.Introduction

Conclusions References
Tables Figures
The aerosol size distribution plots during OH-initiated oxidation of α-pinene under conditions of high and low RH are given in Fig. 3.The particle number concentration decreases as the particles grow in size due to condensation, coagulation, and particle wall-loss to the surface of the chamber, resulting in a shift in the mode to ∼ 170 nm at the end of the experiment with no significant difference in the particle size distributions between high and low RH.A similar observation was reported by Bonn et al. (2002) for the OH-initiated oxidation of β-pinene.
Figure 4 shows the concentration of oxidation products from the OH-initiated oxidation of α-pinene under high and low RH conditions at different α-pinene concentrations (200 ppb vs. 100 ppb, Exp.1-3, Table 1).Experiments with low α-pinene concentrations (100 ppb) show a clear dependence on RH, with formation of higher relative concentrations of almost all acids under dry conditions (RH 14-15 %).The dominant acids in the low α-pinene experiments are MBTCA and DTAA, which account for about 5 and 3 %, respectively, of the total SOA mass.Experiments performed with higher α-Introduction

Conclusions References
Tables Figures

Back Close
Full pinene concentrations (200 ppb) show a much less pronounced effect of RH on the acid concentrations.However, the probable explanation for this observation is that the high concentration α-pinene experiments were performed during late October at much lower ambient temperatures (∼ 5 • C), so the difference between high RH (43 %) and low RH (∼ 15 %) conditions was more pronounced for low concentration α-pinene experiments.In addition, increased condensation at the lower ambient temperatures during the high concentration α-pinene experiments could partly explain the generally higher concentration of acids relative to the low concentration α-pinene experiments.Increased condensation of pinonic acid from the gas-phase during experiments at lower ambient temperatures would also contribute to the significantly lower MBTCA concentration, since less pinonic acid would be available for OH-initiated oxidation in the gas phase (Szmigielski et al., 2007).Furthermore, as the low concentration αpinene experiments were performed on clear sunny days in July-September, higher OH concentrations would explain the significantly higher MBTCA concentration during these experiments relative to the high concentration α-pinene experiments performed in late October.Although precursors suggested in previous studies (i.e., pinic acid, terpenylic acid, and DTAA, Yasmeen et al., 2010) for the dimer esters are present in the aerosols generated in OH-initiated oxidation experiments, no dimer esters were observed.

Ozonolysis of α-pinene: effect of O 3 concentration
Figure 5 shows the NO, NO 2 , O 3 , α-pinene and SOA data from an AS seeded ozonolysis experiment (Exp.4, Table 1) in which the effect of O 3 level on the formation of dimer esters was examined.As no NO was added to the outdoor chamber, concentrations of NO and NO 2 were close to the detection limit of the NO x monitor during the entire experiment.Once α-pinene is injected into the two outdoor chambers, O 3 concentration drops and SOA formation is initiated.Due to a higher O 3 -to-α-pinene ratio, the rate of SOA formation is much higher in the high O 3 chamber relative to the low O 3 chamber.The higher SOA formation rate is also evident in the particle size distribution data col-32540 Introduction

Conclusions References
Tables Figures

Back Close
Full The UPLC/ESI-HR-Q-TOFMS analysis of ozonolysis SOA in Fig. 7 shows only small differences in the chemical composition between the seeded and non-seeded experiments.Overall, the O 3 -initiated oxidation of α-pinene results in several carboxylic acids, with pinic acid being dominant, followed by terpenylic and hydroxy-pinonic acids (∼ 3.2 %, 2 %, and 1.8 % of SOA mass, respectively).Four dimer esters were identified in SOA generated during ozonolysis of α-pinene; pinyl-diaterebyl ester (MW 344), pinyl-diaterpenyl ester (MW 358), pinonyl-pinyl ester (MW 368), and a dimer ester with MW 388 previously observed by Gao et al. (2010).Pinyl-diaterpenyl ester is the dominant dimer esters, constituting about 1 % of the SOA mass.Relative to OH-initiated oxidations, the concentration of the second-generation OH-oxidation product MBTCA is significantly lower in the O 3 -initiated oxidation experiments because of the absence of OH during night-time and added OH scavenger.In addition, almost no DTAA is formed during the dark ozonolysis of α-pinene indicating that DTAA is formed only in the presence of OH-radicals.The four dominant carboxylic acids (pininc acid, terpenylic acid, hydroxy-pinonic acid and pinonic acid) show a slightly higher concentration in SOA sampled from the high O 3 chamber.In addition, pinyl-diaterpenyl ester (MW 358) and the MW 388 dimer also show increased concentration with the higher O 3 level, indicating that elevated O 3 concentration may increase the formation of these compounds from ozonolysis of α-pinene.

Effect of SOA mass on dimer ester formation
Table 1 and Figs to that for SOA collected from the outdoor dual chamber ozonolysis experiments, once again demonstrating that increased SOA mass does not facilitate the formation of the dimer esters from α-pinene.In addition, since the indoor chamber experiment is conducted at very low NO x conditions, it is concluded that the formation of dimer esters proceeds without the presence of NO x , in line with previous investigations (Kristensen et al., 2013).
From Fig. 8 it is clear that the formation of the four dimer esters occurs only during O 3 -initiated oxidation of α-pinene.Only small differences in yield are observed in the low and high SOA mass O 3 -initiated oxidations, indicating that the formation of the dimer esters in the ozonolysis experiment in Fig. 7 is not due to higher SOA mass.The slightly higher relative concentration of pinyl-diaterpenyl ester (MW 358) and the MW 388 dimer ester in the low SOA experiment (Fig. 8) can be explained by the higher ozone-to-pinene ratio, consistent with a similar effect in the high and low O 3 level experiments (Fig. 7).

Effect of aerosol acidity on ozonolysis
The concentration of diaterpenylic acid (DTA) is low (< 0.2 % of SOA mass) in all experiments.Since DTA is tentatively proposed as a precursor of the pinyl-diaterpenyl ester (MW 358) (Yasmeen et al., 2010), one would expect these compounds to be closely correlated.The low concentration of DTA could therefore be hypothesized to explain Introduction

Conclusions References
Tables Figures

Back Close
Full the relatively low yield of the dimer ester (0-1.6 % of SOA mass) relative to carboxylic acids (7-12 % of SOA mass) in all experiments.If DTA were formed through the acid-catalyzed hydrolysis of terpenylic acid, increased aerosol acidity would be predicted to enhance the formation of DTA and thus, pinyl-diaterpenyl ester formation if the mechanism of dimer ester formation was aerosol-phase esterification.Figure 9 shows the NO, NO 2 , O 3 , α-pinene and SOA data from experiment 9 (Table 1) in which the effect of acidity on dimer ester formation was investigated.After the injection of α-pinene, the SOA mass increases simultaneously in both chambers, reaching a maximum of 94 and 78 µg m −3 in the neutral and acidic chambers, respectively.Figure 10 shows the particle number size distribution of aerosol from the acidic and neutral chambers during the experiments.Prior to the injection of α-pinene, the seed concentration, particle size distributions in the acidic and neutral chambers are similar.However, once α-pinene is injected, a significantly higher particle number is produced under neutral conditions than under acidic conditions.In addition, particle growth under neutral conditions is significantly more rapid than under acidic conditions.Despite differences in the SOA mass yield under acidic and neutral conditions, UPLC/ESI-HR-QTOFMS analysis shows similar relative concentrations of acids, including DTA, and dimer esters (Fig. 11).Our finding that formation of DTA is not enhanced under acidic conditions calls into question the proposed acid-catalyzed formation of this compound (Claeys et al., 2009).Based on the results, DTA and DTAA do not seem to be correlated and probably originate via different routes.In addition, increased aerosol acidity does not seem to enhance the formation of the dimer esters significantly.These results are in accordance with the finding by Gao et al. (2004) that preexisting seed was not required for the formation of oligomers and that aerosol acidity did not result in significant increase in the intensities of smaller oligomers, such as the MW 358 dimer ester.Introduction

Conclusions References
Tables Figures

Back Close
Full  The aerosol size distributions during OH-initiated oxidation following the first αpinene injection under conditions of high and low RH are given in Fig. 13a and following the second α-pinene injection, in Fig. 13b. Figure 13b shows that following the second injection of α-pinene and ozonolysis, the particle size distribution in both chambers changes significantly.In the low RH chamber, the mode shifts from 170 nm (the end-point of the OH-initiated oxidation experiment) to around 300 nm, indicating an increased SOA growth due to condensation of ozonolysis oxidation products onto preexisting particles.In the high RH chamber, the particle size generally increases, but distribution appears bimodal in character, with the smaller diameter particles predominating.The significant increase in concentration of smaller particles (D p < 300 nm) would be indicative of new particle formation under high RH conditions due to nu-Introduction

Conclusions References
Tables Figures

Back Close
Full cleation following the O 3 -initiated oxidation of α-pinene.The number concentration of particles with diameters between 10-300 nm produced from ozonolysis of α-pinene has been reported to increase at higher RH and has been ascribed to the presence of water vapor affecting the rate of production or the character of the nucleation species (Jonsson et al., 2006).
As described above, ozonolysis following a second injection of α-pinene into the high RH chamber containing α-pinene SOA from OH-initiated oxidation resulted in an increase in concentration to ∼ 8500 particles cm −3 (Fig. 13b).O 3 -initiated oxidation of α-pinene with pure AS as the pre-existing seed aerosol resulted in an increase to ∼ 120 000 particles cm −3 (Fig. 6a).The ∼ 14-fold increase in total particle number suggests that seed composition could significantly affect new particle formation.Although pure AS seed particles initially have a smaller mode (∼ 70 nm) than seed particles from the OH-oxidation of α-pinene (∼ 170 nm), the total surface area of α-pinene SOA coated AS particles from OH oxidation (2.2 × 10 8 nm 2 cm −3 ) is lower than that of the pure AS seed (4.2 × 10 8 nm 2 cm −3 ).Thus, the aerosol from OH oxidation of α-pinene does not appear to suppress nucleation by virtue of increased partitioning of ozonolysis products onto a larger total surface area.The smaller increase in particle formation observed in Fig. 13b relative to pure AS seed in Fig. 6a could be explained by the presence of an organic surface film consisting of products of OH-initiated α-pinene oxidation on the aqueous AS droplets initially present.As new SOA is formed from the second injection of α-pinene, the organic film could allow for increased condensation of nucleated species thus reducing the formation of new particles through nucleation.
A similar effect has been observed by Kamens et al. (2011), who showed that higher initial seed aerosol from background rural air reduced the nucleation of particles from photooxidation of toluene in an urban hydrocarbon environment with NO x in natural sunlight.Increased condensation of SOA onto the organic-coated seed aerosol might also be explained by differences in viscosity.As SOA from the OH-initiated oxidation α-pinene condenses onto the AS seed aerosol, water uptake by the seed aerosol is enhanced.Prenni et al. (2001) found that some carboxylic acids retain water to very Introduction

Conclusions References
Tables Figures

Back Close
Full  (2000) found that glutaric acid and pinonic acid, in general, enhance water sorption of AS.The increased water uptake by AS due to the presence of organics, such as the carboxylic acids identified in this study, reduces the viscosity of the particles allowing newly formed SOA to partition more effectively into the preexisting particles (Renbaum-Wolff et al., 2013).The increased partitioning of the gas-phase oxidation products to the particles results in a lowering of concentration of gas-phase compounds required for nucleation, hence reducing the formation of new particles, consistent with Fig. 13b.
To confirm that the large increase in particle formation observed in the ozonolysis of α-pinene in the presence of pure uncoated AS seeds arises from nucleation, ozonolysis of identical concentrations of α-pinene was carried out without the addition of AS seed particles.Figure 6b shows the particle size distribution for the chamber ozonolysis of identical concentrations of α-pinene without preexisting AS seed particles at high (∼ 180 ppb) and low (∼ 90 ppb) O 3 levels.Even in the absence of AS seed aerosol, ozonolysis of α-pinene results in significantly higher SOA mass and particle number concentration compared to the experiments involving α-pinene SOA coated AS seed (Fig. 13b).Furthermore, higher particle number concentration is observed in the non-seeded ozonolysis experiment (Fig. 6b) compared to the AS seeded experiment (Fig. 6a), indicating that the presence of AS seed reduces the particle number from α-pinene ozonolysis, likely due to increased condensation.Thus, pure AS seed does not facilitate particle formation from α-pinene ozonolysis, and does not explain the increased particle formation relative to ozonolysis in the presence of SOA generated by condensation of OH-oxidation of α-pinene on AS.By comparison, it is clear that the SOA coated AS particles present in the chamber after OH-oxidation of α-pinene (Fig. 13) result in a significant greater decrease in particle number concentration following α-pinene ozonolysis than that of pure AS (Fig. 6a).This indicates that the pure AS seed aerosol may be less effective in absorbing SOA than AS particles coated with α-pinene SOA.The lower affinity of the α-pinene oxidation products for the pure AS seed aerosol limits the condensation of organics in the aerosol phase resulting in an in-Introduction

Conclusions References
Tables Figures

Back Close
Full creased gas-phase concentration of oxidation products ultimately leading to the formation of new particles through nucleation, which may explain the ∼ 14-fold higher particle number concentration observed in the pure AS ozonolysis experiments (Fig. 6a).
Another explanation for the decreased particle formation observed during the second injection of α-pinene compared to the pure AS ozonolysis experiments could be the presence of organic acids in the gas-phase.Kamens et al. (2005) showed that the presence of formic acid resulted in a decreased particle number concentration during ozonolysis of α-pinene and assigned this to the scavenging of sCI in the gas-phase by formic acid.
Figure 14 shows the relative concentration of acids and dimer esters observed in aerosols from the experiments in which ozonolysis occurred following the injection of αpinene after depletion of α-pinene by OH oxidation.Similar to the observation in Fig. 4, SOA formed under low RH conditions from the OH-initiated oxidation of α-pinene show a higher concentration of organic acids, such as pinonic acid, hydroxy-pinonic acid, terpenylic acid and pinic acid, compared to SOA formed under high RH conditions.
A higher concentration of organic acids in the gas-phase in the low RH chamber prior to the second injection of α-pinene could explain the differences in the particle size distributions shown in Fig. 13b, as higher concentration of gas-phase organic acid may result in a more effective scavenging of sCI, thus reducing the formation of new particles in the low RH chamber compared to the high RH chamber.
From Fig. 14 it is clear that the composition of the SOA changes significantly following the second injection of α-pinene, with increasing relative concentrations of pinic acid, pinonic acid, and hydroxy-pinonic acid.In contrast, the concentration of both MBTCA and DTAA is significantly lower in the SOA collected after the second injection of αpinene (about 1.5 and 1 % of total SOA mass, respectively).Absence of OH in the chamber during night-time is the most likely explanation for this observation.In addition, increased SOA formation from O 3 -initiated oxidation of α-pinene and increased condensation of semi-volatile compounds due to lower night-time temperatures contribute to reducing the normalized MBTCA and DTAA concentrations.Increased condensation Introduction

Conclusions References
Tables Figures

Back Close
Full at lower night-time temperatures may also explain the significantly larger fraction of semi-volatile compounds, such as pinonic acid, pinic acid, and hydroxy-pinonic acid in SOA collected following the second α-pinene injection.As indicated above, increased condensation of pinonic acid at lower temperatures also contributes to the observed decrease in MBTCA.
The low MBTCA and DTAA concentrations observed following the second injection of α-pinene indicates that no significant gas-phase oxidation of already present oxygenated VOCs (OVOC) from the preceding OH-initiated oxidation experiment is occurring.Formation of OH from the ozonolysis of α-pinene following the second injection could allow for increased oxidation of OVOC in the chamber, thus increased condensation and SOA mass.However, since no increase in MBTCA and DTAA is observed in SOA collected after the second injection, further oxidation by OH does not seem to be the reason for the increased SOA formation observed during ozonolysis.
In contrast to SOA produced during OH-oxidation, dimer esters were identified in SOA generated during ozonolysis following the second injection of α-pinene.The composition of the SOA collected after the second injection of α-pinene shows a clear RH dependence, with highest relative concentrations of all four dimer esters in SOA formed at high RH.The presence of dimer esters in SOA collected after the second injection indicates that the dimers are formed only during ozonolysis of α-pinene in accordance with results from individual OH-and O 3 -initiated oxidation experiments shown in Fig. 8.
To summarize, O 3 -initiated oxidation of α-pinene is shown to produce higher SOA mass compared to OH-initiated oxidation.Furthermore, the reaction of α-pinene with O 3 results in a more complex SOA due to the formation of dimer esters in addition to acids.The formation of the dimer esters is enhanced at higher RH, indicating a possible RH effect on the chemical composition of SOA from α-pinene ozonolysis.Increased new particle formation (Fig. 13b) correlates with increased formation of dimer esters (Fig. 14), suggesting that the gas-phase formation of the polar, high-molecular weight compounds results in new particle formation by homogenous nucleation.Introduction

Conclusions References
Tables Figures

Back Close
Full

Atmospheric relevance
Formation of the dimer esters identified in this study is currently hypothesized to result from aerosol-phase esterification of carboxylic acids.In this study, the formation of dimer esters has been shown to occur only during ozonolysis of α-pinene and not through OH-oxidation, suggesting that these dimers originate via the gas phase reaction of a stabilized CI formed by ozonolysis of α-pinene.This mechanism is supported by the increase in the formation of dimer esters observed at higher RH, explained by increased stabilization of the CI, and previous work, by Witkowski and Gierczak (2013), showing that sCI may react with aldehydes to form higher molecular weight esters.By contrast, particle-phase esterification of carboxylic acids would be expected to increase at lower RH.Furthermore, recent findings have shown that particle-phase Fischer esterification is kinetically unfavourable under atmospheric conditions (Birdsall et al., 2013;DePalma et al., 2013).Yasmeen et al. (2010) observed several dimer esters during warm, summer nights in K-Puszta, Hungary, and suggested that higher temperatures (> 24 • C) and high concentrations of pinic acid during night-time could facilitate the formation of dimers.In this study, dimer esters from α-pinene are observed in ozonolysis experiments performed at temperatures as low as 10 • C, proving that dimers may form at low temperatures.Since increased pinic acid concentration is observed in all O 3 -initiated oxidation experiments (∼ 3 % of SOA mass) relative to OH-oxidation (∼ 1 % of SOA mass), it is not possible to rule out formation of the dimer esters from pinic acid in the particle phase.However, the observation that no trace of dimer esters is observed in the OH-initiated oxidations, despite the presence of pinic acid and other dimer precursors, indicates that pinic acid does not seem to be the controlling factor in formation of the dimer esters, and furthermore rules out formation through previously suggested esterification.This conclusion is confirmed in a study showing a low correlation between pinic acid and the pinyl-diaterpyl ester in ambient measurements (Kristensen et al., 2013).Introduction

Conclusions References
Tables Figures

Back Close
Full The suggested formation of dimer esters through a sCI, originating from ozonolysis of α-pinene would explain the observed diurnal variations of the dimer esters at both K-Puszta, Hungary, and Blodgett forest, USA (Yasmeen et al., 2010;Kristensen al., 2013).Since OH-initiated oxidation of α-pinene dominates during daytime, because of the high concentration of OH radicals and the fast reaction rate of OH with α-pinene (Atkinson et al., 2006), no dimer esters are formed.However, during night-time, when the concentration of OH radicals is low and α-pinene is still emitted from the terrestrial system, O 3 -initiated oxidation of α-pinene predominates leading to the formation of the dimer esters.
Gas-phase formation could also explain the increased concentration of dimer esters observed at the higher ambient temperatures of K-Puszta, Hungary, and Blodgett forest, USA, which would increase gas-phase concentrations of semi-volatile pinic and hydroxy-pinonic acids, and thus, enhance gas-phase reactions forming high-MW compounds, such as the dimer esters observed in this study.Higher ambient temperatures might further enhance the gas-phase formation of dimer esters by increasing the reaction rate of the sCI.Due to the high molecular weight and polar nature of the dimer esters, gas-phase formation could result in the homogenous nucleation observed in Fig. 13b.Based on the findings of this study, RH may influence the chemical composition of SOA from both OH-initiated oxidation and ozonolysis of α-pinene.The observed effect of increased RH on the formation of α-pinene oxidation products indicates that changes in climate may alter the chemical composition of SOA by facilitating the formation of higher MW compounds, such as the dimer esters.As this study indicates that dimer esters are formed only through ozonolysis of α-pinene, they may be useful tracers for O 3 -initiated oxidation chemistry, and could therefore serve as indicators of increased anthropogenic influence on SOA formation.Introduction

Conclusions References
Tables Figures

Back Close
Full

Conclusions
Dimer esters from α-pinene appear to be formed through ozonolysis and not through OH-initiated oxidation, making these potentially useful tracers for atmospheric ozone chemistry and anthropogenic pollution.Despite the presence of the dimer precursors, no dimer esters were observed in SOA from OH-oxidation of α-pinene.This observation along with the increased particle fraction of dimer esters at higher RH and no significant influence of particle acidity, indicates that the dimer esters are not formed through the currently postulated particle-phase esterification of carboxylic acids.This conclusion is supported by kinetic studies (DePalma et al., 2013).We suggest that the formation of the dimer esters through gas-phase reactions of the stabilized CI, can explain the ozone dependence and the fast formation of dimer esters observed in previous studies.Furthermore, gas-phase formation of the dimer esters may result in homogenous nucleation, explaining the increased new particle formation during α-pinene ozonolysis at higher RH.Similar concentrations of dimer esters in low and high SOA mass experiments show that the dimer esters observed in this and previous smog chamber studies are not formed as a consequence of high SOA mass loading.Significantly less particle formation was observed from ozonolysis of α-pinene in the presence of AS seed particles coated with products of OH + α-pinene oxidation than in the presence of pure AS seed.This effect is attributed to an increased condensation of newly formed oxidation products from the ozonolysis of α-pinene onto the less viscous SOA-coated AS seed that depletes the gas-phase concentrations of oxidation products, and thus, prevents new particle formation through nucleation.This conclusion underlines the influence of seed particles in smog chamber studies, and emphasizes the effect of both composition and physical state of the seed aerosols on new particle formation.Further research is warranted to understand the gas-phase formation of the dimer esters, which may offer insight into the reactivity and role of Criegee intermediates in the formation of SOA from α-pinene.Introduction

Conclusions References
Tables Figures

Conclusions References
Tables Figures

Conclusions References
Tables Figures

Conclusions References
Tables Figures

Back Close
Full Discussion Paper | Discussion Paper | Discussion Paper | Discussion Paper | Discussion Paper | Discussion Paper | Discussion Paper | Discussion Paper | Discussion Paper | Discussion Paper | Discussion Paper | Discussion Paper | Discussion Paper | Discussion Paper | Discussion Paper | Discussion Paper | Discussion Paper | Discussion Paper | -initiated oxidation of α-pinene: effect of RHThe results of the investigation of OH-initiated oxidation of α-pinene under initially high-NO concentrations and different RH conditions are shown in Figs.2-4.Figure2shows the time-dependence of concentrations of NO, NO 2 , O 3 , α-pinene and SOA.The concentration of NO decreases following an initial injection of 100 ppb α-pinene to both chambers by reaction between NO and hydroxyperoxy radicals (RO 2 ) formed from OH-initiated oxidation during the day (e.g.Arey et al., 2001).Once sufficient NO 2 has been formed, photolysis of NO 2 initiates O 3 formation in the chambers.Prior to the increase in O 3 concentration, most of the injected α-pinene has been consumed by reaction with OH with formation of 21-22 µg m −3 of SOA under low RH (14-15 % initial RH) conditions and 22-28 µg m −3 under high RH (27-46 % initial RH) conditions (Exp.
Discussion Paper | Discussion Paper | Discussion Paper | Discussion Paper | Discussion Paper | Discussion Paper | lected from both chambers during both seeded and non-seeded experiments (Fig. 6a and b), showing higher particle number concentration in the high O 3 chambers.
Figure 6.A) Particle size distributions in low (80ppb, blue) and high (170-ppb, red) ozone chamber following the injection of α-pinene (50ppb) along with size distribution of AS seed Fig. 6. (A) Particle size distributions in low (80 ppb, blue) and high (170 ppb, red) ozone chamber following the injection of α-pinene (50 ppb) along with size distribution of AS seed particles prior to the injection (black) (Exp.4, Table1).(B) Particle size distributions in low (90 ppb, blue) and high (180 ppb, red) ozone chamber following the injection of α-pinene (50 ppb) without the addition of AS seed (Exp.5, Table1).

2.4 Filter sample extractions and UPLC/MS analysis
Table 1).As observed in individual OH-and O 3 -initiated oxidation experiments (Figs. 2 and 5), although only 50 ppb of α-pinene is added during the second injection following sunset, the formation of SOA from ozonolysis is more rapid and yields higher SOA concentrations, ranging from 37-68 and 35-64 µg m −3 of SOA in the low and high RH chambers, respectively.Saathoff et al. (2009) has reported a negative temperature dependence of SOA yields from ozonolysis of α-pinene, and thus, the lower temperature during the second injection in our experiments might contribute to higher production of SOA mass during nighttime.As shown in Table 1, SOA formation under both high and low RH conditions is similar in all experiments with oxidation by either OH or O 3 , indicating that the RH has minimal effect on the SOA mass yield from both oxidants.